The objective of this study was to develop high quality and yield medicinal fungi. Fomitopsis pinicola (Swartz. Fr.) Karst., were cultured for 35 days and the mycelia dry weight at the level of 6.55 g/l. The yield of polysaccharide of Fomitopsis pinicola was 0.38 g/l, and the ethanolic extraction was about 17% (ethanolic extraction/mycelia dry weight).The biological assay included polysaccharides, and ethanolic extract. The MTT test showed no toxicity to ECV (human vascular endothelial cell) cells up to a concentration of 1000 μg/ml. Interferon-γ inducible protein 10 (IP-10) induced by interferon-γ plays an important role in cell inflammation. By the expression of IP-10, the present result showed no effect for the polysaccharides of Fomitopsis pinicola. Among the ethanolic extract of Fomitopsis pinicola inhibited IP-10 protein release by 58% at concentration of 100 μg/ml.