Purpose: The purpose of this study was to understand the effect of carbohydrate ingestion on metabolic gene expressions in human skeletal muscle during post-exercise recovery. Methods: Six subjects participated in two experimental trials. In one of the experimental trials, they ingested a high carbohydrate mixed meal immediately after a cycle ergometer for 60 minutes at 70-75% VO2 peak. In the other one, they were not allowed to eat for 3 hours after the same exercise protocol. The muscle samples of these subjects were obtained from vastus lateralis with a needle biopsy technique 3 hours later after exercise. These muscle samples were analyzed using the oligonucleotide microarray. Results: The result showed that the muscle samples from those who took a carbohydrate mixed meal had 57 genes up-regulated and 41 genes down-regulated compared to the samples from the fasting condition. Conclusion: The ingestion of a high carbohydrate mixed meal during post-exercise recovery was suggested to alter metabolic gene expressions in human skeletal muscle.