This study on adding different molecular weight of aloe into chitosan and silk fibroin and nanofiber membrane was prepared by electrospinning. The fineness and water absorption properties of membranes were investigating. The results showed the fibers more uniformed and fineness increasing with increase of concentration of aloe. The nanofiber membranes made from mixture of silk fibroin/aloe/ chitosan were environmental and available for biomaterials. The water absorption and diffusion coefficient are increase with aloe.