Nodule Cultures of Paulownia x Taiwaniana

瘤狀細胞團培養在植物組織培養的再生系統、自動化的微體繁殖與植物化學物的生産上，已被視爲具有相當潛力的應用價值。本研究首次建立台灣泡桐的瘤狀細胞團培養並可幾代培養一年以上。首先將葉片培植體培養在MS培養基添加3mg/L BA以誘導生長旺盛的癒合組織細胞團，再移植於1/2MS液體培養基添加各3mg/L之NAA與BA，經在130rpm轉速下的迴轉震盪器培養6週後，瘤狀細胞團隨即被誘導出來。瘤狀細胞團經幾培養3個月及1年後，其生長趨勢可用細胞沈澱法及電導度法加以測定。這兩種測定法所得的結果相當一致，但電導度法較爲快速、簡單且重複性高。觀察瘤狀細胞團在各不同生長時期組織切片，可明瞭瘤狀細胞之分化及維管束組織形成的過程。在前數個繼代培養的瘤狀細胞團具有再生芽體的能力，但隨著繼代次數的增加其再生能力即逐漸喪失。

關鍵字

瘤狀細胞團培養；台灣泡桐

並列摘要

Nodule cultures with potential applications in regeneration strategies, automated micropropagation, and in vitro phytochemical production in many plants have been reviewed. The lst nodule cultures of Poulownia x taiwaniana were established and maintained for more than 1 y by regular subculturing. Vigorous callus clumps were induced from leaf explants cultured on 1/2MS medium containing 3 mg/L IBA and 3 mg/L BA. Nodule cultures were produced after leaf-derived calli were transferred into MS liquid medium containing 3mg/L each of IBA and BA, and placed on a reciprocal shaker at 130 rpm for 6 wk. Cell growth cycles of the 3 mo-old and 1-y-old subcultures were determined by using settled cell volume and electrical conductivity. Results obtained from these 2 measurements were consistent with each other. Conductivity appeared to be rapid, simple, and reproducible. Histological changes of cell differentiation and vascular organization were also investigated at different growth phases of cultures. Nodules maintained organogenic capability in the lst few subcultures, but lost it in successive cultures.

並列關鍵字

nodule cultures ； Paulownia x taiwaniana

國際替代計量

Nodule Cultures of Paulownia x Taiwaniana

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