Glass Formation by Galactopinitol with Other Sugars and Their Ability to Protect Phospholipid Vesicles from Drying Damage

甲基肌醇半乳吡喃糖和其他糖類的玻璃質化形成及其保護人工脂囊膜免於乾燥受損的能力

沈里國(Li-Kuo Shen) ； 簡慶德(Ching-Te Chien) ； 林讚標(Tsan-Piao Lin)

《台灣林業科學》 15卷3期 (2000/09) Pp. 293-301

https://doi.org/10.7075/TJFS.200009.0293

摘要

甲基肌醇半乳吡喃糖(Galpi)，萃取自薩爾瓦多銀合歡種子，與蔗糖、棉子糖(raffinose)和水蘇糖(stachyose)等組合，以瞭解糖類在乾燥下之玻璃質化形成和人工脂囊膜的穩定性。棉子糖、水蘇糖和甲基肌醇半乳吡喃糖有良好的玻璃質化特性，其玻璃質化轉移溫度(Tg)分別為67.4、66.4和33.4℃。上述這些糖類的玻璃質化轉移溫度比葡萄糖和蔗糖高出很多。甲基肌醇半乳吡喃糖和蔗糖以0.3對1重量濃度混合比例，其玻璃質化轉移溫度在0℃以上，且近似於相同比例之寡糖類（棉子糖或水蘇糖）和蔗糖混合，顯示甲基肌醇半乳吡喃糖和寡糖類在玻璃質化形成上扮演重要的角色。甲基肌醇半乳吡喃糖和蔗糖混合對人工脂囊膜保護作用，其結果與棉子糖（或水蘇糖）和蔗糖混合之保護作用相同。上述這些糖混合對人工脂囊膜之保護作用百分比約為38-48%。由結果推論銀合歡種子在乾燥時，甲基肌醇半乳吡喃糖類似寡糖類，也許可防止細胞在乾燥下受到損害。

關鍵字

耐旱性 ； 甲基肌醇半乳吡喃糖 ； 寡糖 ； 玻璃質化轉移溫度 ； 脂囊膜

並列摘要

A galactopinitol (Galpi), O-α-D-galactopyranosyl-(1→1)-3-O-methyl-D-chiro-inositol, together with sucrose, raffinose, and stachyose were extracted from seeds of Leucaena leucocephala (Lam.) de Wit and characterized for glass formation and phospholipid vesicle stabilization during dehydration. Raffinose, stachyose, and Galpi were found to have good glass forming properties, and their glass transition temperatures (Tgs) were 67.4, 66.4, and 33.40℃, respectively. The Tgs were much higher than those of glucose and sucrose. The Tg for a mass ratio mixture of Galpi and sucrose of 0.3/1 was greater than 0 and was similar to that of the same ratio mixture of raffinose to sucrose, indicating that Galpi as well as oligosaccharides plays an important role in glass formation. The mixture of Galpi plus sucrose appeared to protect phospholipid vesicles during dehydration and rehydration to the same degree as did raffinose or the stachyose plus sucrose mixture. The percent protection (leakage of isocitrate) of vesicles provided by sucrose, Galpi, raffinose, and stachyose was in the approximate range of 38 to 48%. The results suggest that Galpi may prevent cellular collapse during the desiccation of leucaena seeds and function much the same as oligosaccharides.

並列關鍵字

desiccation tolerance ； galactopinitol (Galpi) ； oligosaccharide ； glass transition temperature (Tg) ； phospholipid vesicle