Ectomycorrhiza Enhanced the Cold-Acclimation Growth and Freeze Tolerance of Scots Pine (Pinus Sylvestris L.)

外生菌根增進歐洲赤松（Pinus sylvestris）的冷馴化生長和耐凍性

奧特根蘇仁布倫(Burenjargal Otgonsuren) ； 李明仁(Ming-Jen Lee)

《台灣林業科學》 28卷2期 (2013/06) Pp. 97-111

https://doi.org/10.7075/TJFS.201306_28(2).0004

摘要

歐洲赤松（Pinus sylvestris, Scots pine）為蒙古重要的經濟用材來源，且廣泛應用於人工造林計畫。吾等先前的研究曾證實Phialocephala fortinii可以和歐洲赤松苗木形成共生組合外生菌根。本研究顯示，接種Phi. fortinii顯著地增進正常及冷馴化處理的歐洲赤松苗木的生長、生物量、及其根、莖、葉中礦物質（磷、鉀、鈣、鎂、鈉、氮）的含量。此外，在健化及冷馴化處理下，接種Phi. fortinii的歐洲赤松苗木的脯胺酸含量顯著高於未接種者。接種及未接種的歐洲赤松苗木經冷馴化處理，隨之分別於-12、-14、-16、-18及-20℃進行耐凍性試驗7天後，並培養於12±2℃生長箱14天。接種及未接種的歐洲赤松苗木針葉的半致死溫度分別為-12及-15℃。相一致地，接種及未接種的歐洲赤松苗木的半致死溫度分別為-14及-18℃。結果顯示P. fortinii不但可以和歐洲赤松苗木形成外生菌根，並能顯著地增進其生長、養分獲得、脯胺酸含量、及耐凍性。

關鍵字

Phialocephala fortinii ； Pinus sylvestris ； 外生菌根 ； 耐凍性 ； 脯胺酸

並列摘要

Scots pine (Pinus sylvestris) is an economically important source of timber in Mongolia and has been widely used in reforestation programs. Our earlier study showed that Phialocephala fortinii was capable of forming symbiotic ectomycorrhizal associations with Scots pine seedlings. In this study, Phi. fortinii inoculation significantly increased the growth, biomass, and mineral (P, K, Ca, Mg, Na, and N) contents in roots, stems, and needles of Scots pine seedlings under normal and cold-acclimation conditions. Furthermore, the proline content of inoculated Scots pine seedlings was significantly higher than that of non-inoculated ones after hardening and cold-acclimation treatments. The inoculated and non-inoculated Scots pine seedlings were cold-acclimated and subsequently subjected to freezing tolerance tests at -12, -14, -16, -18, and -20℃ for 7 d, and then cultivated at 12±2℃ for 14 d. Values of the temperature for 50% mortality (LT50) of needles of non-inoculated and inoculated Scots pine seedlings were -12 and -15℃, respectively. Consistently, respective LT50 values of seedlings of non-inoculated and inoculated pines were -14 and -18℃. In addition to Phi. fortinii effectively forming ectomycorrhiza with Scots pine seedlings, this study demonstrated that Phi. fortinii significantly improved the growth, nutrition acquisition, proline content, and freeze tolerance of Scots pine.

並列關鍵字

Phialocephala fortinii ； Pinus sylvestris ； ectomycorrhiza ； freeze tolerance ； proline