Leaf Anatomy in Cross-section Features of 29 Genotypes of Oil Tea from Hainan Island and Their Systematic Significance

海南島29份油茶基因型葉片解剖及其系統學意義

金亦佳(Yijia Jin) ； 閔曉航(Xiaohang Min) ； 袁德義(Deyi Yuan) ； Joseph Masabni ； 熊歡(Huan Xiong) ； 鄒鋒(Feng Zou)

《台灣林業科學》 36卷1期 (2021/03) Pp. 21-34

https://doi.org/10.7075/TJFS.202103_36(1).0002

摘要

Oil tea belongs to the Camellia genus, which is mainly distributed in southern China and is one of the four woody edible oil plants in the world. Recently, we identified some genotypes of oil-tea plants on Hainan Island. However, we did not know whether these plants belonged to C. oleifera. This study compared 11 leaf anatomical characters for 29 genotypes of oil tea from Hainan Island to 1 genotype of C. oleifera from Hunan Province, China. A principal component analysis and cluster analysis were conducted using transformed data for quantitative and qualitative characters of the leaf anatomy to explain their relationships. Results indicated that leaf thickness ranged 230.45~460.79 μm. Diameters of the midrib vessels ranged 349.63~729.85 μm. Palisade tissues consisted of 1 or 3 layers of arranged columnar cells, accounting for 25.10 or 41.47%, respectively, of the leaf thickness. Similarly, spongy tissues accounted for 42.14~72.49% of the thickness of the leaves. The area of the adaxial epidermis ranged 384.75~1643.90 μm^2, and the perimeter of the adaxial epidermal cells varied from 55.74 to 183.05 μm. Leaves classified according to genotype were divided into 2 groups using the principal component and cluster analyses. We observed differences in leaf anatomical characters of genotypes collected between the 2 regions, suggesting that oil tea from Hainan Island has a close relative relationship with C. oleifera and belongs to Camellia sect. Oleifera. Leaf morphological characteristics could provide important information for genotype identification of these oil-tea plants in the future.

關鍵字

Camellia Sect. Oleifera ； leaf anatomy ； principal component analysis ； cluster analysis

並列摘要

油茶屬山茶屬植物，是世界四大木本食用油料植物之壹，主要分佈在中國南方。近年來，我們對海南島油茶進行了基因型鑒定。然而，我們不知道這些植物是否屬於普通油茶。本研究比較了海南島29份油茶基因型與1份普通油茶基因型的11個葉解剖特徵。利用葉片解剖學定量和定性性狀的轉換數據進行主成分分析和聚類分析，以解釋二者之間的關系。結果表明：葉厚230.45～460.79 μm，中脈導管直徑349.63～729.85 μm，柵欄組織由1～3層排列整齊的柱狀細胞組成，分別占葉厚的25.10～41.47％。同樣，海綿狀組織占葉片厚度的42.14～72.49％。近軸葉面積384.75～1643.90 μm^2，近軸面細胞周長55.74～183.05 μm。根據基因型劃分，利用主成分分析和聚類分析法將30份分為兩組。我們觀察了兩個地區不同基因型葉片解剖特徵的差異，認為海南島油茶與普通油茶有著密切的親緣關系，屬於油茶組。油茶葉片形態特徵可為今後油茶的基因型鑒定提供重要資訊。

並列關鍵字

油茶組 ； 葉片解剖 ； 主成分分析 ； 聚類分析