Purpose: This study aimed to examine whether the nursing practice grade can serve as a predictor of pass rate for the first attempt of the Registered Nurse (RN) Examination of baccalaureate nursing graduates. Methods: A retrospective study was conducted to collect data from the Nursing Practice Management System in one university. Data covered the grades of various nursing practice courses of students who graduated from 2015 to 2019. All the students had completed the required nine nursing practice courses. The outcome was pass/ fail in the first attempt of the RN examination. Results: Among a total of 260 students, most of them were Taiwanese (95%) and female (82.3%). The pass rate of the RN examination on the first attempt was 93.5%. The nursing practice grades have significant difference on the RN examination between pass and fail. A high grade in Maternal and Infant Nursing Practice was a predictor of success in the RN examination. The cut-off score of 82.5 [approximately a grade point average (GPA) of B] for Maternal and Infant Nursing Practice was determined with an area of 0.77 (95% confidence interval: 0.67-0.88) under the receiver operating characteristic curve. Conclusions: Identifying the nursing practice grade could be a predictor of the RN examination pass rate, can be used to screen students at risk of failing the RN examination and to early begin tutoring intervention to increase the RN examination pass rate.