菊科及大戟科植物抗發炎活性分析及篩選

Analysis and Screening of Anti-inflammatory Activity on Compositae and Euphorbiaceae Plants

羅英妃(Ying-Fei Lo) ； 王靜瓊(Ching-Chiung Wang) ； 李美娟(Mei-Jiuan Lee)

《植物種苗》 15卷1期 (2013/03) Pp. 53-66

摘要

本研究以19種菊科植物及18種大戟科植物之50%甲醇萃取物來進行生物活性分析。實驗結果顯示，利用細菌脂多醣誘發鼷鼠RAW264.7細胞產生NO之抑制發炎活性評估，在參試之菊科植物中，以台灣款冬之抗發炎活性最佳，約92.56%，其抑制NO生成的IC50為27.59 μg/ml。對於癌細胞株（T24、HeLa及HepG2）之細胞毒性指數值（CI%）分別為14.68%、3.67%及37.82%，對正常細胞（3T3細胞株）之細胞毒性指數值約30%。在參試之大戟科植物中，以白飯樹之抗發炎活性佳，約74.42%，且不會影響RAW264.7巨噬細胞之存活率，對癌細胞株T24、HeLa及HepG2之毒性較低，僅分別為25.54%、30.73%及35.33%，但其對正常細胞（3T3細胞株）具有強烈細胞毒性，細胞毒性指數值約96.6%，綜合上述結果，台灣款冬及白飯樹具有較佳之抗發炎活性，未來若欲開發利用，仍需進行相關毒性試驗評估。

關鍵字

菊科植物 ； 大戟科植物 ； 細胞毒性 ； 抗發炎活性

並列摘要

In this study, 19 kinds of Compositae and 18 kinds of Euphorbiaceae plants were extracted by 50% MeOH for bioactivity assay. The anti-inflammatory activity of plant extracts was performed by the inhibition of lipopolysaccharide-induced NO production in RAW264.7 macrophages. Petasites formosanus Kitamura had the highest anti-inflammatory activity (about 92.56%) of Compositae plants tested. However, it showed cytotoxicity on normal cell line (3T3) (CI% about 30%), but not on cancer cell lines (T-24, HeLa, and HepG2, (CI% are 14.68%, 3.67% and 37.82%, respectively). In Euphorbiaceae plants extracts, Fluggea virosus Roxb. Willd had the higher anti-inflammatory activity(about 74%) of plants evaluated, this plant also showed strong cytotoxicity on normal cell line (3T3) (CI% about 96.6%), but not on cancer cell lines (T-24, HeLa, and HepG2) (CI% are 25.54%, 30.73% and 35.33%, respectively). In according to the above results, Petasites formosanus Kitamura and Fluggea virosus Roxb. Willd had great anti-inflammatory activity, but the toxicity should be concerned for the development in the future.

並列關鍵字

Compositae ； Euphorbiaceae ； Cytotoxicity ； anti-inflammatory activity