【論文摘要】The Effect of High Intensity Interval Training and Moderate Intensity Continuous Training on Function of Natural Killer Cell

【論文摘要】高強度間隔式運動訓練與中等強度連續式運動訓練對於自然殺手細胞功能之影響

陳柏瑜(Po-Yu Chen) ； 王鐘賢(Jong-Shyan Wang)

《物理治療》 45卷2期 (2020/06) Pp. 123-124

https://doi.org/10.6215/FJPT.202006.O05

摘要

Background and Purpose: Exercise has a great impact on the normal function of the immune system. We have known prolonged-intensive exercise training can depress immunity, but regular-moderate intensity exercise is beneficial. The relationship between exercise and immunity can be demonstrated in ＂J Form Curve＂. Natural killer cell, large granule lymphocyte, which accounts for 10% of lymphocytes in the peripheral blood of humans. Natural killer (NK) cell plays an important role in the innate immune system, possessing related toxic hormones to fight target cells. We focus on the way that NK secretes perforin and Grazyme B to alter membrane-targeted death (BID) ligand into truncated BID in the cancer cell, initiating a series of death pathway. Comparing to the moderate intensity continuous training (MICT) group, we hypothesize that the amount of perforin and Granzyme B has much more significant increasing in the high intensity interval training (HIIT) group. On efficiency of electron transport chain and mitochondria O_2 consumption, we assume that HIIT is greater than the MICT group. Methods: Thirty-six healthy sedentary males were randomized to engage in either HIIT (3-min intervals at 40% and 80% VO_2 reserve, n = 12) or MICT (sustained 60% VO_2 reserve, n = 12) on a bicycle ergometer for 30 min/day, 5 days/week for 6 weeks, or to a control group that did not engage in exercise training (n = 12). All subjects have done the Graded exercise test before the training. Extracting NK cells, we observe intact NK cell O_2 consumption by Oxygraph-2K, and the fluorescent-stained perforin and granzyme B in perforated NK cells by cytometer. Results: First, the increasing numbers of NK cell in HIIT are 2.5 times of the MICT group. Second, on mitochondria O_2 consumption, HIIT group has greater reserve capacity than the MICT group. On BHI index, the HIIT group have significant improvement than the MICT group. Next on perforin fluorescent intensity, HIIT group show significant increasing than the MICT. On Granzyme B fluorescent intensity, both groups increase in the similar degree. Conclusion: Comparing to the MICT, HIIT program has greater impact on increasing numbers of natural killer cells. Also, HIIT make mitochondria in NK cell have higher O_2 consumption efficiency. HIIT has greater influence on increasing number of perforin and granzyme B. Clinical Relevance: HIIT has been proven a great intervention toward heart failure patient. We hope that we will extend the use of HIIT program to benefit human health.