高粱酒糟機能性成分萃取

本研究分別探討溶劑及超臨界二氧化碳萃取參數對高粱酒糟之長鏈脂肪醇、植物固醇、總酚化合物及單寧等成分萃取之影響。試驗結果顯示，每公斤高粱酒糟之長鏈脂肪醇萃取量以乙醇、乙酸乙酯及正己烷之348、322及275mg較高；植物固醇部分則以正己烷、乙醇及乙酸乙酯之323、317及311mg較佳。總酚化合物及單寧之萃取量以50%乙醇水溶液最高，萃取物之DPPH自由基清除能力亦以50%乙醇水溶液萃出物較佳。在超臨界二氧化碳萃取方面，萃取壓力之影響並不明顯，而萃取溫度為主要影響因子，萃取溫度在65℃以下時，提高萃取溫度可增加長鏈脂肪醇、植物固醇、總酚化合物及單寧之萃取量，於65℃/379 bar萃取3小時其長鏈脂肪醇及植物固醇萃取量分別為273mg及516mg，經分離槽區分後，萃出物之長鏈脂肪醇含量可提升至2.16%，較傳統正己烷萃取僅0.94%為佳。

關鍵字

超臨界二氧化碳萃取；高粱酒糟；長鏈脂肪醇

並列摘要

Solvent and supercritical carbon dioxide were used to extract the functional compounds, including polycosanols, phytosterols, total phenolic compounds and tannins, from sorghum distillery residues (SDR), respectively. Results showed that using ethanol, ethyl acetate and n-hexane as extraction solvents, the extraction yields of policosanols were 348, 322 and 275 mg/Kg SDR, and those of phytosterols were 317, 311 and 323 mg/Kg SDR. Using 50% ethanol as extraction solvent, extraction yields of total phenolic compounds (TPC) and tannins were the highest among all extraction, and the DPPH scavenging ability of the extract was also higher than those obtained by using other solvents. By using supercritical carbon dioxide to extract SDR, an increase in temperature would result in a higher yields of policosanols, phytosterols, total phenolic compounds and tannins, but this effect was leveled off when the temperature is higher than 65℃. The policosanols and phytosterols extraction yields could reach 273 mg and 516 mg, respectively, at the condition of 65℃/379 bar supercritical carbon dioxide extraction for 3 h. After separation, the policosanols content of the extract was 2.16% higher than that of n-hexane solvent extract with 0.94% of policosanols content.

並列關鍵字

Supercritical carbon dioxide extraction ； Sorghum distillery residues ； Policosanol

國際替代計量

高粱酒糟機能性成分萃取

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