芭樂籽溶劑萃取物對小鼠初代脾臟細胞細胞激素分泌之影響

Effects of Guava Seed Solvent Extracts on Cytokine Secretions by Murine Primary Splenocytes

連思惠(Szu-Hui Lien) ； 顧琪玫(Chi-Mei Ku) ； 林金源(Jin-Yuarn Lin)

《臺灣農業化學與食品科學》 50卷5&6期 (2012/12) Pp. 271-280

https://doi.org/10.6578/TJACFS.2012.030

摘要

為了解芭樂籽之免疫調節潛力，本研究以購入之芭樂果實收集其種子進行試驗。先將芭樂籽在40℃烘箱中乾燥後，再將芭樂籽磨成粉末，並將芭樂籽粉末分別加入四倍去離子水、80%乙醇或正己烷進行萃取，萃取液減壓濃縮去除有機溶劑後冷凍乾燥，取得芭樂籽的水、80%乙醇和正己烷萃取物，以分析其對小鼠初代脾臟細胞細胞激素分泌之影響。試驗結果顯示，水及80%乙醇萃取物在156、625及2,500 μg/mL等濃度，正己烷萃取物在78、156及312 μg/mL等濃度，對脾臟細胞無顯著細胞毒性；取樣品（非細胞毒性濃度）與脾臟細胞共同培養48小時，分析脾臟細胞抗發炎細胞激素介白質（interleukin，IL）－10和促發炎細胞激素IL－1β、IL－6及腫瘤壞死因子-α（TNF-α）分泌量的變化，顯示添加芭樂籽水萃取物有增加BALB／c雌鼠脾臟細胞分泌細胞激素之能力，具有免疫刺激活性；添加80%乙醇萃取物，則同時降低抗發炎和促發炎細胞激素的分泌，推測具有抗發炎及免疫抑制作用；正己烷萃取物則未見免疫調節潛力。綜合本研究結果，證實芭樂籽的水萃取物及80%乙醇萃取物在動物體外試驗具有不同的免疫調節活性，將來或可應用於不同的免疫調節目的。

關鍵字

細胞激素 ； 芭樂籽萃取物 ； 脾臟細胞

並列摘要

To understand whether guava seeds have immunomodulatory potential, guava (Psidium guajava L.) fruit was purchased and its seeds were isolated for study. Isolated guava seeds were dried in an oven at 40℃ and then milled into powder. The powder samples were extracted using four volumes of de-ionized water, 80% ethyl alcohol or n-hexane. The organic solvent in the filtrates were evaporated using a rotary evaporator and freeze dried to obtain guava seed water extracts (GSWE), eighty percent ethyl alcohol guava seed extracts (AGSE) and n-hexane guava seed extracts (HGSE), which were used to assess their effects on cytokine secretions by murine primary splenocytes in vitro. The results showed that GSWE and AGSE at 156, 625 and 2,500 μg/mL, as well as HGSE at 78, 156 and 312 μg/mL did not significantly affect cell viability, reflecting their optimal non-cytotoxic concentrations to splenocytes. The apparent non-cytotoxic doses of GSWE, AGSE and HGSE were selected to treat splenocytes for 48 hours. Anti-inflammatory cytokine interleukin (IL)-10 and pro-inflammatory cytokines levels including IL-1β, IL-6 and tumor necrosis factor alpha (TNF-α) in the cell culture supernatants were determined. GSWE addition increased cytokine secretions by splenocytes, suggesting that GSWE might have an immunostimulatory activity. AGSE addition decreased both anti-inflammatory and pro-inflammatory cytokines, suggesting that AGSE might have anti-inflammatory and immunosuppressive effects. HGSE addition did not significantly affect splenocyte cytokine secretion profiles, suggesting that HGSE had little immunomodulatory potential. In conclusion, this study provides evidence that GSWE and AGSE exhibit differential immunomodulatory activities in vitro. Our results suggest that GSWE and AGSE may be of value for different immunomodulatory purposes in the future.

並列關鍵字

Cytokines ； Extracts of guava seeds ； Splenocytes