台灣鋏蠓（Forcipomyia taiwana）誘引物質之實驗室篩選研究

Laboratory Evaluation of Chemical Stimulants for Trapping Forcipomyia taiwana (Shiraki; Diptera: Ceratopogonidae)

羅怡珮(Yi-Pey Luo) ； 簡良芬(Liang-Fen Jian)

《台灣昆蟲》 40卷2期 (2020/05) Pp. 144-156

https://doi.org/10.6662/TESFE.202005_40(2).003

摘要

本研究開發台灣鋏蠓（Forcipomyia taiwana）嗅覺誘引測試平台，設計雙向嗅覺誘引裝置進行定量檢測化學物質對台灣鋏蠓的誘引效果。以9～12日齡、已交尾的雌成蟲進行測試，試驗結果顯示以碳酸氫銨（ammonium bicarbonate; 1.0 × 10^(-1) g/mL）、芥酸（erucic acid; 1.0 × 10^(-8) g/mL）及乳酸鈉（sodium DL-lactate solution; 1.3 × 10^(-1) g/mL）和碳酸氫銨（5 × 10^(-2) g/mL）混合物，誘引指數可以達到以人體手掌誘引效果的61～68%。本研究開發的台灣鋏蠓雙向嗅覺誘引裝置，可簡便進行新化學誘引物質的篩選，並希望能應用於開發大量誘殺台灣鋏蠓雌成蟲的技術，做為另一種防治台灣鋏蠓的策略，減少雌蟲吸血擾人的困擾。

關鍵字

台灣鋏蠓 ； 雙向嗅覺誘引裝置 ； 嗅覺刺激物 ； 誘引率 ； 誘引指數

並列摘要

To assess the attractant efficacy of chemical stimulants, a dual-port olfactometer was used to quantitatively investigate the response of a biting midge, Forcipomyia taiwana (Shiraki; Diptera: Ceratopogonidae), to host-derived volatiles. Tests were conducted on the copulated female biting midges aged 9 to 12 days to determine their responses to human odors and chemicals. The most attractive chemical stimulants to F. taiwana were ammonium bicarbonate solution (10^(-1) g/mL), erucic acid solution (10^(-8) g/mL), and a combined solution of sodium lactate (1.3 × 10^(-1) g/mL) and ammonium bicarbonate (5 × 10^(-2) g/mL). In terms of sensitivity and simplicity, the bioassay conducted was especially suited to analyzing this biting midge; it is a useful tool for searching for new attractive compounds. This work will hopefully facilitate further research on the chemical ecology of F. taiwana. The potential use of related attractants for biting midge control is discussed herein.

並列關鍵字

Forcipomyia taiwana ； dual-port olfactometer ； odor stimulus ； catch rate ； catch index