篩選具益生菌活性之鵝源乳酸桿菌

Screening of the Probiotic Activity of Lactobacillus spp. from Geese

邱敏蕙(Min-Huei Ciou) ； 余章游(Chang-You Yu) ； 蘇耀期(Yao-Chi Su) ； 賴治民(Jyh-Mirn Lai)

《臺灣獸醫學雜誌》 35卷4期 (2009/12) Pp. 253-259

https://doi.org/10.7009/TVJ.200912.0253

摘要

乳酸桿菌廣泛存於自然界中以及動物體內，已知對宿主的消化系統具有多重益處，因此在人或者豬、雞等動物的相關資訊已被深入探討，然而在鵝及鴨的益生菌研究仍十分缺乏，故本研究目的是藉由體外試驗(in vitro)來篩選出有潛力鵝的益生菌。所有分離株皆進行病原菌抑制試驗（Salmonella Typhimurium與Escherichia coli）、抗菌劑感受性試驗（十二種抗菌劑紙錠）、耐酸性試驗(pH2.0的MRS broth)、上皮吸附試驗（鵝嗉囊上皮細胞）、耐膽鹽試驗（2%的MRS broth）與分子生物鑑定，僅LAG81通過上述試驗，在分子生物鑑定方面顯示LAG81與sp. T059的相似度為99%。以上連續試驗結果，迴腸所分離出的LAG81最具有成為雛鵝益生菌的潛力。

關鍵字

篩選 ； 益生菌 ； 鵝 ； 乳酸桿菌

並列摘要

Lactobacillus exists in natural environment and gastrointestinal systems of animals. Because it is well known that it benefits to the host's alimentary tract in many ways, many publications, especially in humans, pigs and chickens, have been published and discussed deeply. However, just a few were reported in geese and ducks. The aim of this study was to select potential probiotics from geese in vitro. Lactobacillus suspected isolates were tested by pathogen inhibiting tests, using Salmonella Typhimurium and Escherichia coli, antimicrobial susceptibility tests, using 12 antimicrobial paper disks, acid tolerance test on Lactobacilli MRS Broth with pH 2.0, adhesive ability of epithelial test, using geese crop epithelial cells, bile salt tolerant test, using Lactobacilli MRS Broth with 2% bile salt and molecular biological identification. Only the LAG81 strain passed through all assays and the sequence of its molecular biological features were similar to Lactobacillus sp. T059, whose relation was 99%. According to the results, the LAG81 strain isolated from geese ileum was a high potential probiotic for geese.

並列關鍵字

screening ； probiotics ； geese ； lactobacillus