- 期刊
針對兩種不同廠牌血瓶實驗比較臨床常見厭氧菌與小兒血液培養基去抗生素的偵測時間與陽性回覆率
Comparison with Two Blood Culture Systems BACT/ALERT and BACTECTM 240 Antibiotic Effect on Time to Detection and Recovery Rate
摘要
血液感染的診斷是醫院緊急且重要的試驗，快速而準確的血液培養偵測系統扮演舉足輕重的角色。對細菌檢驗實驗室來說，要從接受抗生素治療的臨床病患血液中分離出致病菌非常需要，且厭氧菌血液培養檢驗在厭氧菌敗血症的診斷是非常重要，因此細菌檢驗室將評估二種廠牌BACTEC(上標 TM) 9240 System （A廠牌），BacT/Alert(上標 TM)System（B廠牌）的小兒去抗生素血液培養瓶吸附抗生素的效果與偵測時間是否有差異。 選取血液感染中常見厭氧臨床菌株，配製成1.5×10^1 CFU/ml 及1.5×10^2 CFU/ml菌株懸浮液分別接種於A,B二種廠牌厭氧血液培養瓶，並以重複三次試驗方式進行評估；選取血液感染常見菌株及常用抗生素配合藥物動力學，將不同濃度之抗生素及ATCC菌株懸浮液分別接種於二種廠牌小兒去抗生素血液培養瓶，並以三次重覆方式進行評估。 在本次實驗中，由結果顯示A廠牌提供細菌室優異之厭氧菌株回復率與偵測陽性時間且A廠牌血瓶可有效吸附抗生素縮短偵測陽性時間，帶給臨床準確快速的血液培養報告。
並列摘要
To avoid the influence of pre-analytical steps, this study was performed using sterile blood spiked with defined loads of microorganisms as inoculum. Time-to-Detection (TTD) was estimated for the most frequently encountered bacteria comparing two commercially available blood culture systems, BD BACTECTM 9240 (Becton Dickinson) and BacT/ALERTTM (Organon Teknika). The effect of the most widely used antibiotics on TTD was evaluated on both systems. TTD was measured with antibiotics at their trough and at increasing concentrations. The results show that the BACTEC PLUS system recovers more pathogens with shorter time to detection than the BacT/ALERT FAN system when beta-lactam antibiotics (Vancomycin, Ampicillin, Cefotaxime) are present at their respective trough concentration corresponding to parenteral therapy. The two systems seem to be equally efficient when Cefotaxime and Gentamicin are used; in the case of Vancomycin, Ampicillin, Cefoxitin, BACTEC seems more effective than BacT/ALERT.
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