The purpose of the research was to investigate the relationships among the career plateau, lack of job resources, job stress and burnout in nurse staffs. A cross-sectional survey with the purposive sampling by questionnaires form the nurse staffs of two Metropolitan Hospitals in central and southern Taiwan. The valid samples were 290 (72.5%) in 400 samples. The results showed that the fully mediated effect of job stress affected the relationship between the career plateau and job burnout. The partially of mediated effect of job stress impacted the relationship between the lack of job resources and job burnout. The results of the study may provide medical managers to develop strategies of reference for the establishment of the promotion system and the allocation of resources.