This paper aimed to explore how to deepen the education of nano-literacy on students. We survey the different nano-literacy competence from students. The study conditions in students' nano-literacy competence is the main purpose in our study, therefore, we designed several indexes of nano-literacy competence. Furthermore, for improving and compiling the matters of teaching in general education courses, we observe the nano-literacy teaching effectiveness and student-learning satisfaction in Nanotechnology general education courses by questionnaires. This study focus on general education courses in National Dan University. This paper has chosed the period of time in ninety-nine academic year, 2011 as our research. And we divided our investigation into three stages: beginning term, middle term and final term, by using questionnaires of ”Nano-literacy teaching effectiveness and student-learning satisfaction in National Ilan University”. Then we compare each stage of learning in this semester, and analyzing if there have several significantly different strides among the research time from students.