Virus-tested Lycoris aurea Plants from Apical Meristems of Adventitiously Regenerated Shoots

金花石蒜試管內之不定芽再生莖其生長點培養建立無病毒健康苗

黃麗春(Li-Chun Huang) ； You-Ming Chang ； 劉邦基(Pan-Chi Liou) ； 許華欣(Wha-Shin Hsu) ； 陳金枝(Chin-Chu Chen) ； 黃寶蓮(Bau-Lian Huang) ； 張清安(Chin-An Chang)

《Botanical Studies》 47卷2期 (2006/04) Pp. 163-166

摘要

金花石蒜又名金色蜘蛛百合為台灣原生花卉，本研究先以試管內雙鱗片培養建立試管再生系統，誘導不定芽大量分化和繁殖，不定芽長大成苗後再切取其小於0.1毫米高之微小莖頂生長點（不帶有葉原體），促使其發育抽長成莖、及繁殖、發根、馴化後，移植管外於隔離溫室繼續生長茁壯。以酵素抗体分析法(ELISA)鑑定種植一年後及連續四年共五年追蹤四種金花石蒜potyviruses病毒（LPV、LyMMV和LVT）及胡瓜嵌紋病毒(CMV)，確定無上述四種病毒，即無LPV、LyMMV、LVT、CMV之毒素病存在。自莖頂生長點培養獲得之健康無性系，現已成為原原種(Fundation stock)建立大量田間種植及繁殖。

關鍵字

胡瓜嵌紋病毒 ； 石蒜微斑紋病毒 ； 石蒜Y病毒 ； 石蒜T病毒 ； 生長點培養 ； 無病毒驗證植株

並列摘要

Adventitious shoots were first regenerated in vitro from twin-scale sections of virus-infected Lycoris aurea Herb. cv. 'Golden spider lily'. Their apical meristems were then excised and cultured to redevelop shoots. Explants were not larger than 0.1 mm and were limited to the dome and subjacent stem, without leaf primordia. The redeveloped shoots were multiplied, rooted, hardened, and acclimatized, before transfer to an insect-free greenhouse. ELISA assays were performed for three lycoris potyviruses (lycoris potyvirus, LPV; lycoris mild mottle virus, LyMMV; and lycoris virus Taiwan, LVT) and for cucumber mosaic virus (CMV). Assays after one year and re-assays in the subsequent three years revealed no LyMMV, LPV, or CMV in the meristem-derived plants or in their clonal progeny. The virus-tested plants are now being increased as foundation stock.

並列關鍵字

Cucumber mosaic virus ； Lycoris mild mottle virus ； Lycoris potyvirus ； Lycoris virus Taiwan ； Lycoris ； Meristem culture ； Virus tested plants