移動相中添加S-β-環糊精在逆相液相層析上分離萘酚和萘甲醚位置異構物

Separation of Naphthol and Methoxynaphthalene isomers by RPLC Using S-β-cyclodextrin as Mobile Phase Additives

俞仁渭(Jen-wei Yu) ； 林宣慧(Xuan-hui Lin) ； 張書文(Shu-wen Chang) ； 林振興(Chen-hsing Lin)

《高雄師大學報：自然科學與科技類》 47期 (2019/12) Pp. 39-52

摘要

本研究在移動相中添加S-β-環糊精於甲醇與水混合溶液，利用RP-C18靜相管柱分離萘酚和萘甲醚位置異構物，並探討移動相中甲醇含量對這些分析物分離的影響。經由層析圖可知，添加1g／L S-β-環糊精於甲醇與水適當比例的移動相中，利用RP-C18靜相管柱可以基線分離萘酚和萘甲醚位置異構物。本研究利用理論計算得到萘酚和萘甲醚個別與β-環糊精之錯合物結構，分析其中氫鍵作用力之差異，推測萘酚與S-β-環糊精之間的氫鍵作用力在滯留次序上扮演重要角色。

關鍵字

逆相液相層析 ； S-β-環糊精 ； 分析物 ； 位置異構物 ； 理論計算

並列摘要

The separation of naphthol and methoxynaphthalene isomers was investigated by high-performance liquid chromatography on a RP-C18 stationary phase using S-β- cyclodextrin (S-β-CD) as mobile phase additives. Effects of methanol content of mobile phase on the separation of analytes were examined. The Chromatograms of the naphthol and methoxynaphthalene isomers show that baseline separations were achieved on a commercial RP-C18 stationary phase using S-β-CD in the appropriate methanol content of mobile phase. In this study, the structure of the complex of naphthol and methoxynaphthalene isomers with β-CD was calculated by theoretical calculation, and the difference of hydrogen bonding force was analyzed. The hydrogen bonding force between naphthol and S-β-CD plays an important role in the retention order.

並列關鍵字

Reversed Phase Liquid Chromatography ； S-β-cyclodextrin (S-β-CD) ； Theoretical Calculation ； Analytes

參考文獻

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