The purpose of this study was to explore the differences of fitness, learning motivation and physical education attitude of undergraduate female student in different types of university. The participant was undergraduate female student in north of Tao-yuan. Used questionnaire survey and random Sampling method to proceed the research. There were 600 questionnaires sent out and retrieved 540 effective questionnaires. The participants were 166undergraduate students and 374 four-year institutes of technology students. The result was as followed: 1. There were differences between different types of university students in standing long jump, 800m walk-run, BMI, Satisfaction of learn motivation, total learn motivation, affect of physical education attitude, behavior intention and total attitude. 2. The score in total fitness, total motivation and total behavior of regular exercise group was higher than those of none exercise group and low exercise group. 3. There was positive relation between fitness, affect of physical education attitude and behavior relevance, and there was positive relation between learning motivation and physical education attitude.