- 期刊
Complement-Mediated Hemolysis by Subunits of IgM Antibody
IgM抗體小單元對補體作用產生之溶血
摘要
兔子對抗二硝基醛（Dinitrophenol）的免疫球蛋-M（Immunoglobulin M, IgM）抗體是用專一元提煉法從抗血清分離出來。IgM抗體含有五個相同的小單元（Subunit）經由雙硫鍵（Disulfide bond）環連起來。這種小單元稱的IgMs，利用暖和的還原作用（Reduction）及烴化作用（Alkylation）使雙硫鍵體折斷而把IgMs彼此分散開來，然後這些散開的IgMs小單元再用重複凝膠濾過法（Gel filtration）把鏈間雙硫鍵（Inter chain disulfide bond）完整及折斷的IgMs再彼此分離出來。定量測定結果顯示這兩種IgMs都具有與補體（Complement）作用產生之溶血能力（Hemolytic Activity）。不過，這些鏈間雙硫鍵完整的IgMs之溶血能力只有原來IgM抗體之溶血能力之二千分之一，同時約相等於IgG抗體之溶血能力。鍵間雙硫鍵折斷之IgMs的溶血能力又比鏈間雙硫鍵完整的IgMs要小2.5倍。
關鍵字
無資料並列摘要
IgMs* subunits with intact inter-μ chain disulfide bonds were isolated from specifically purified rabbit IgM anti-DNP antibody after mild reduction and alkylation, and separated from IgMs with broken inter-μ chain disulfide bonds by recycling gel filtration on Sepharose-4B. Quantitative measurements of the ability of these subunits to mediate hemolysis by complement show that both are hemolytically active. The hemolytic activity of the IgMs with intact inter-μ chain disulfide bonds is comparable to that of specifically purified IgG anti-DNP antibody, and is about 2,000 times less than that of intact IgM antibody. Loss of the inter-μ chain disulfide bonds leads to a further 2.5-fold reduction in activity.
並列關鍵字
無資料延伸閱讀
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