This study was to investigate the differences of production traits on rice by two kinds of nitrogen fertilizer application, which one was applied 120 kg per hectare and the other was 60 kg. The results indicated that the leaf area index and nitrogen contents in the straw and grain of rice for applying 120 kg were larger than those for 60 kg, but the net assimilative rate and starch contents in the straw and grain were to the contrary. On the other hand, the reduction and translocation of nitrogen in straw and grain applying for 120 kg after heading to ripening stage were higher than those for 60 kg, but the results of starch were to the opposite effects. In addition, the leaf area index, the starch contents in straw, the nitrogen and starch contents in grain and the reduced contents of nitrogen in straw in the first crop were larger than those in the second crop. But the reduced contents of starch in the straw were reverse. By the way, the translocations of nitrogen and starch between the first crop and the second crop were similar.