不同栽培方式對薏苡根系代謝物化學指紋圖譜之影響

Study on the Chemical Fingerprints of Root Extracts of Coix lacryma-jobi in Different Culture Conditions

陳裕星(Yu-Hsin Chen) ； 張金元(Jin-Yuan Chang) ； 吳浩銘(Hao-Ming Wu)

《臺中區農業改良場研究彙報》 127期 (2015/06) Pp. 15-26

摘要

薏苡為具有豐富營養價值與機能性成分作物，薏苡根也是傳統藥材，具有豐富的機能性成分，包括6-methoxy-benzoxazolinone （6-MBOA）及酚酸類化合物等，具有清熱，利濕，健脾等功效。本研究探討水耕、霧耕以及土耕方式對薏苡根系生長與代謝物含量之變化，研究中以超音波輔助甲醇萃取方式，建立薏苡根系於260、280、330、360nm波長下之HPLC化學指紋圖譜，作為整體成分特徵與含量比較之依據，另以280 nm分別分析不同處理之薏苡根系，比較特殊代謝機能性成分的差異。結果顯示薏苡水耕和霧耕根系重量差異不顯著，代謝物組成以極性中等酚酸類成分為主，水耕根系中含有最高含量的6-MBOA，其次則為霧耕，其他成分在不同耕作方法中並無明顯的趨勢。

關鍵字

薏苡根 ； 化學指紋圖譜 ； 水耕 ； 霧耕

並列摘要

Job's tears (＂Coix lacryma-jobi＂) is a nutritious cereal crop that enriches in functional metabolites such as 6-methoxy-benzoxazolinone (6-MBOA) and phenolic acids. Root of Job's tears is a traditional Chinese medicine which can alleviate fever, promote dieresis and toning spleen. In this project, we investigated the growth and metabolites composition of roots of Job's tears among hydroponic, mist and soil culture. Root samples were subjected to ultrasonic assisted methanol extraction. Overall features of extracts were characterized using 260, 280, 330 and 360 nm HPLC absorption spectrums. Chemical fingerprinting was established using HPLC at 280 nm as basis for comparison of chemical profiles and for specific metabolites quantification. The results indicated that roots weight is higher in soil culture, but did not differ significantly between hydroponic and mist culture. The content of 6-MBOA in roots was higher in hydroponic culture. There is no significant trend on profiles variation among different culture methods.

並列關鍵字

Job's tears root ； chemical fingerprinting ； hydroponic culture ； mist culture