Agriculture aims to enhance not only better quality, safety of agricultural products but also environment protection and leisure function of agriculture business. Bacillus thuringiensis (Bt) is considered to be one of the most potential biopesticides to replace some chemical pesticides. For example, the development of indigenous Btk E911, that can control Diamond-back moth, cabbage lopper, cabbage worm, and tussock moth, we have been according to the requirements of registration, use the microbial technology and biotechnology to select and identify new isolates. When we find one isolate has the best bioactivity, we not only try to pilot scale up, but also test the animal toxicity. Then, this champion isolate and the techniques, including basic mass production process, quality control process and field test results, transfer to cooperative company to become a commercial product.