尼龍植絨、人造絲與棉花拭子採集微生物檢體的效能

拭子常規用於採集微生物檢體，由於各種不同種類的拭子是以不同材質所製造而成、本研究測試三種不同材質的拭子包括尼龍植絨（CMP nylon flocked swab, CMP efSwab）、人造絲和棉花拭子，藉以評估採集微生物檢體的效能（微生物吸附和釋放能力），測試的菌種為環境中常見的微生物包括：Staphylococcus aureus、Escherichia coli、Bacillus subtilis與Candida albicans，測試時，將四種含有濃度1.5×10^3 CFU/mL的菌液分別取100 μL滴在三種拭子上以及滴在9 cm^2無菌塑膠培養皿和金屬的表面上，再以拭子進行採樣，塗抹在trypticase soy agar（TSA）平板培養基上，然後將平板置入35°C一般培養箱培養36－48小時。結果顯示尼龍植絨拭子對四種菌的釋放能力以及在塑膠或金屬表面的微生物樣本採集（吸附）能力最具效能，因此，吾等建議使用尼龍植絨拭子採集臨床檢體或工業廠房之環境微生物監控檢體能獲得較正確的效能。

關鍵字

尼龍植絨拭子；人造絲和棉花拭子；釋放能力；微生物樣本採集

並列摘要

Swabs are routinely used to collect samples for microbiological testing. As there are a variety of swabs made with different materials, we tested the effectiveness of 3 different swabs, including nylon flocked (CMP efSwab), rayon, and cotton swabs, in collecting microbial samples. Four different microorganisms that are commonly found in environments, including Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Candida albicans, were tested. For the tests, 100 μL each of the four microbial suspensions containing 1.5×10^3 CFU/mL was placed directly on each of the three different swabs and on 9 cm^2 sterile plastic Petri dishes and metal surfaces. The samples placed on plastic and metal surfaces were then collected with each of the 3 different swabs. All swab samples were inoculated on trypticase soy agar (TSA) plates and incubated in an incubator at 35°C for 36-48 hours. Results showed that nylon flocked swabs (CMP efSwab) were most effective in releasing microbes from swabs and in collecting microbial samples from plastic or metal surfaces. Therefore, we recommend nylon flock swabs (CMP efSwab) be used to collect samples from clinical specimens or from environments such as industrial factories for microbiological monitoring.

並列關鍵字

Nylon flock swabs ； rayon and cotton swabs ； releasing microbes ； collecting microbial samples

國際替代計量

尼龍植絨、人造絲與棉花拭子採集微生物檢體的效能

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