新興濫用藥物2C-T-2的藥理性質以及用氣相層析質譜法鑑定尿液中的代謝成份

The bioactivity of 2,5-dimethoxy-4- ethylthiophenethylamine (2C-T-2) and its detection in the urine of rats by GC/MS

周秀慧(Shiu-Huey Chou) ； 柳如宗(Ju-Tsung Liu) ； 林立川(Li-Chan Lin) ； 許又仁(You-Ren Shu) ； 邱鈺智(Yu-Chih Chiu) ； 林震煌(Cheng-Huang Lin)

《化學》 63卷1期 (2005/03) Pp. 87-94

https://doi.org/10.6623/chem.2005005

摘要

本文介紹2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2)對老鼠的脾臟及胸腺淋巴細胞內NO活性的影響。研究發現當2C-T-2餵食後，小鼠脾臟的T-和B-淋巴細胞與胸腺的T-淋巴細胞經過活化後，細胞產生NO的量都有明顯的減少。這代表2C-T-2會干擾免疫細胞活性，因為NO的減少表示免疫能力的降低。另一方面，實驗以腹腔皮下注射方式對三隻大鼠給藥(20mg/kg)，並收集注射前後24小時的尿液，運用GC-SIM-MS 的方法進行2C-T-2在老鼠體內的代謝路徑的分析。尿液樣品經過加酸加熱水解後，以乙醚液-液萃取方式萃取代謝物。實驗結果發現四種主要可能的代謝物，並由此推測2C-T-2在老鼠體內的代謝路徑。

關鍵字

一氧化氮 ； 苯乙胺 ； 2C-T-2 ； 代謝作用

並列摘要

The bioactivity of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) on nitric oxide (NO) production and the proliferation of spleen and thymus lymphocytes to mitogen stimulation in mice are reported. NO production by T and B lymphocytes in spleen and T cells in the thymus of mice decreased after the oral administration of 2C-T-2. This indicates that 2C-T-2 intake may perturb both neural and immune activity since a decrease in NO production is indicative of a weakened defense function. On the other hand, a simple and specific method based on gas-chromatography-selected ion monitoring mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2C-T-2 in rats is described. Three male rats were administered 20 mg/kg of 2C-T-2 by intra-peritoneal injection, and 24 h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites are present and the metabolic pathway for 2C-T-2 in the rat is proposed.

並列關鍵字

mitric oxide (NO) ； phenethylamine (PEA) ； 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) ； metabolism