以基質輔助雷射脫附游離及電噴灑游離質譜法分析微生物

Analysis of Microorganisms by Matrix-assisted Laser Desorption/Ionization and Electrospray Ionization Mass Spectrometry

林佳儀(Jia-Yi Lin) ； 何彥鵬(Yen-Peng Ho)

《化學》 65卷2期 (2007/06) Pp. 81-93

https://doi.org/10.6623/chem.2007010

摘要

近來新的質譜離子化方法，如電噴灑游離(ESI)及基質輔助雷射脫附游離(MALDI)出現後，鑑定微生物(micro-organisms)的化學分析快速發展成形，這些方法使微生物的直接鑑定或經少許樣品製備過程後的快速鑑定成為可能。這篇論文提供簡略的電噴灑游離法及基質輔助雷射脫附游離法介紹，及微生物鑑定能力的討論，對蛋白質、脂質及其他化合物的混合物分析亦有些許著墨，這篇文章最後以展望未來發展的觀點作結束。

關鍵字

電噴灑游離 ； 質輔助雷射脫附游離 ； 微生物鑑定 ； 蛋白質 ； 脂質

並列摘要

Characterization of microorganisms is critical for the diagnosis and subsequent treatment of infectious diseases, as well as for the detection of biohazards in the environment. Matrixassisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) have both been used to analyze microorganisms. There are a number of advantages to using MALDI-MS for the direct identification of bacteria, including the high speed and sensitivity of the technique, the simplified mass spectra obtained (predominantly singly charged ions), and the approach tolerance to contaminants. ESI-MS allows on-line detection to be combined with sample purification, concentration, and separation techniques, such as microdialysis, solid phase extraction, liquid chromatography, and capillary electrophoresis (CE). Thus, ESI-MS has become a powerful tool for the analysis of complex systems.

並列關鍵字

Electrospray ionization ； Matrix-assisted laser desorption ionization ； Microorganisms ； Identification