Callus induction and regeneration of potato from shoot tip culture

Umme Zohora Laboney ； Gokul Chandra Biswas ； Mohammad Abdullah-Al-Shoeb ； Md. Abunasar Miah

《International Journal of Agricultural Sciences》 3卷10期 (2013/12) Pp. i+040-045

並列摘要

Two laboratory experiments were performed with potato cv. Granula to investigate callus induction ability and regeneration capability from shoot explants and to study the influence of BAP, IAA and GA_3 on plantlet regeneration from callus. In the first experiment, MS medium with 2.0 mg/l 2,4-D were used to identify the highest callus formation (95.00%) within a minimum number of days (10.00), number of calli /culture (4.75) and weight of callus (0.074 g). However, the second experiment was done for both of shoot and root formation. In case of shoot regeneration, combination of 1.0 mg/l BAP and 0.5 mg/l GA3 showed the highest number of shoots per explants (4.67), shoot initiation (93.33%) at required days (15.00), number of leaves per plantlet (3.67) and longest shoot (5.33 cm). On the other hand, the combination of 1.0 mg/l IAA and 1.0 mg/l GA3 showed the highest number of roots per shoot (3.33) within a minimum number of days (12.33) and longest root (6.33 and 7.33 cm) after 20 and 28 days, respectively. Therefore, it was concluded that the hormonal concentration to effect the callus formation and regeneration. Further attempts have been used for production of disease free potato variety from this study.

並列關鍵字

Callus ； culture medium ； in vitro ； regeneration ； shoot tip