耐高溫放線菌分離及其纖維素分解酵素活性

Isolation of Thermo-tolerant Actinomycetes and Their Cellulase Activities

楊盛行(Shang-Shyng Yang) ； 徐麗嵐(Li-Lan Hsu) ； 魏嘉碧(Chia-Bei Wei)

《中華生質能源學會會誌》 30卷3&4期 (2011/12) Pp. 111-118

https://doi.org/10.6385/JBESC.201112.0111

摘要

由農產廢棄物堆肥及生物肥料「田力寶」中，於50°C下分離出11株耐高溫放線菌，挑選其中四株生長較快之分離株編號40、50、A-2及Dot進行固態培養，測定纖維素分解酵素活性。分離株40及A-2號以液態培養，在CMC為唯一碳源之Mandels-Reese培養時，分離株40號培養五天後菌體量最高為0.19mg/ml，培養液pH值則是先升後降。其纖維素分解機制則是先由外切型纖維素分解酵素（Avicelase）將結晶形的區域由非還原端以纖維雙醣切下後，再由內切型纖維素分解酵素（CMCase）由非結晶形區域從內部任意打斷。葡萄糖苷鍵（β-glucosidic bond）最後由β-葡萄糖苷酵素切斷雙醣間的鍵結。

關鍵字

耐高溫放線菌 ； 纖維素分解酵素 ； β-葡萄糖苷酵素

並列摘要

To investigate the thermo-tolerant actinomycetes in the cellulose degradation during compost preparation, the actinomycetes were isolated from agricultural waste composts and biofertilizer Tan-Li-Pao. There were 11 thermo-tolerant actinomycetes were isolated at 50°C. Isolates No. 40, 50, A-2 and Dot had high growth rates and selected for cellulase activities determination with solid cultivation. Isolate No. 40 had the maximal biomass 0.19 mg/ml in Mandels-Reese broth with CMC as sole carbon source for 5 days cultivation. The culture pH increased first and then decreased gradually to pH 7.10. The cellulose was decomposed first in the crystal area from non-reduced end with Avicelase，and then cleavage the β-glucosidic bond with CMCase. The disaccharide bond was finally cleavaged by β-glucosidase.

並列關鍵字

Thermo-tolerant actinomycetes ； cellulase ； β-glucosidase