斜紋夜蛾細胞株之建立

從斜紋夜蛾蛹體卵巢建立細胞株，定名為SL7A。初級培養時細胞從組織塊呈變形蟲狀分生而出，經繼代培養六十代後，細胞株之外部型態呈圓球形。利用TNM-FH昆蟲細胞培養液添加10%胎牛血清可繼代培養本細胞株。SK2A細胞在25T培養盒培養時，其附著於培養盒底部程度較弱，比其它細胞株如SF21AE更容易進行繼代培養。SL7A細胞株在28℃定溫箱培養時，細胞生長速率呈指數型生長曲線，細胞倍增時間為21.4小時。利用25T細胞培養盒培養本細胞株時，經四天後最高培養之細胞數量可達2×10^7個，約為起始細胞數的20倍。本細胞株染色體呈細小點狀，染色體數量變異很大，最少為63個，最多可達382個，平均為215.5±74.3個。同功異構分析顯示，SL7A細胞株在酯、蘋果酸去氫及乳酸去氫等之蛋白質電泳膠片之泳動速率均與其他四種供試細胞株間有顯著不同。

關鍵字

斜紋夜蛾；細胞株；倍增時間；細胞核型；同功異構酶

並列摘要

A cell line was established from pupal ovaries of the tobacco cutworm, Spodoptera litura. This cell line, designated as SL7A, was grown in TNM-FH insect cell culture medium supplemented with 10% fetal bovine serum. In primary cultures, small numbers of wandering connective-tissue-like cells appeared. After subculturing, cells generally became spherical rather than spindle shaped and adhered to the bottom of the culture plate, forming a monolayer of cells. The growth curve of the cells was Y＝e^(-0.6736＋0.0323x)，r^2＝0.98. The population doubling time during logarthmic growth at 28℃ was 21.4h. The saturated population of cells was approximately 2×10^7 cells in a 25-T flask, representing a 20-fold increase over the initial population in 4 days. Karyotype analysis revealed that the nucleus of SL7A cells contained numerous microchromosomes. The number of chromosomes appeared to follow a normal distribution in the range from 63 to 382 with a mean of 215.5±74.3. Isozyme analysis revealed that the SL7A cell line was characteristically different from 4 insect cell lines.

並列關鍵字

Spodoptera litura ； cell line ； growth curve ； karyotype ； isozyme

國際替代計量

斜紋夜蛾細胞株之建立

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