To evaluate the feasibility of using Jacaranda acutifolia extract as an antioxidant ingredient for cosmetics, a series of antioxidant activity assays were performed on the acetone extracts from its flower. The results indicated that the ethyl acetate soluble fraction (JAFE) of Jacaranda acutifolia flower extracts exhibited the highest DPPH radical scavenging and ferric ion reducing activities. The ethyl acetate soluble fraction (JAFE) and butyl alcohol soluble fraction (JAFB) of the extracts were further separated for constituent identification, and five flavonoid compounds were obtained. The structural related antioxidant properties of above flavonoid compounds were examined. Test results revealed that IC_(50) values of DPPH free radical scavenging capability for these five compounds were ranged from 0.18 to 0.82 mg/mL.