青香藥草抗氧化與黃嘌呤氧化酶抑制能力之探討

The antioxidant activities and xanthine oxidase inhibition effect of herbs

高昱心(Yu-Shin Koh)

指導教授 ： 黃文達 ； 楊棋明

國立臺灣大學/生物資源暨農學院/農藝學研究所/碩士(2018年)

https://doi.org/10.6342/NTU201802909

摘要

隨著現代化的開始，全球的疾病負擔漸漸從傳染性疾病轉移到慢性疾病。慢性疾病是由於快速的都市化、全球性的不健康生活方式以及人口老齡化所驅動的。爲了生命，必須在生活中提早去管理自我的健康、預防疾病，因此開發天然保健植物這領域也漸漸受到人們的關注。黃嘌呤氧化酶 (xanthine oxidase, XO) 是體內合成尿酸的關鍵，抑制黃嘌呤氧化酶的活性便能減少尿酸生成。從天然物中萃取能夠治療高尿酸血症又無副作用的植物化學物質已成為許多研究開發的目標。植物含有許多功效成分，可通過抑制黃嘌呤氧化酶活性，而減少體內尿酸的合成，減少高尿酸血症形成。本研究篩選具有降尿酸功效之植物原料，可供作開發成相關之保健食品或醫療藥品。比較在四種氮肥等級 (0 kg ha-1、20 kg ha-1、50 kg ha-1及100 kg ha-1) 的營養管理模式下，其葉片反射光譜與抑制黃嘌呤氧化酶活性的關聯性。目前篩選100種香藥草保健功效，發現奧勒岡、麝香木、齒葉薰衣草、甜萬壽菊、檸檬桉及胡椒薄荷抑制黃嘌呤氧化酶能力均優於紫蘇，其中已於一般生活飲食使用的為奧勒岡、甜萬壽菊及胡椒薄荷。於精準栽培之遙測光譜分析技術試驗中，結果顯示胡椒薄荷、甜萬壽菊、奧勒岡及甜薰衣草黃嘌呤氧化酶抑制率與常態化差異植生指數NDVI 705,750 有極顯著的相關性，同時香草處理氮肥等級與NDVI broad (660~705, 750~800) 間之相關性亦達顯著。因此未來於香草量化生產時，可以藉由非破壞植生指數輔助氮肥管理與掌握產品原料抑制黃嘌呤氧化酶能力，可提供良好農業規範之參考。未來可發展精緻農業栽培天然保健植物，鼓勵產學合作研發具有特色的產品。

關鍵字

保健植物 ； 抗氧化力 ； 抑制黃嘌呤氧化酶活性 ； 葉片反射光譜

並列摘要

Extraction of natural chemicals for the treatment of hyperuricemia and no side effects of plant chemicals has become the goals of research. Xanthine oxidase (XO) is a key enzyme that catalyzes the last step in the conversion of purines to uric acid. Plants contain many active ingredients, which can inhibit the activity of XO, and reduce the formation of uric acid in blood and then prevent hyperuricemia. This study screened the plant material with uric acid effect, for the development of related health food or medical drugs. The leaf reflectance spectra were correlated with the inhibition of xanthine oxidase activity in the nutrient management mode of four nitrogen fertilizers (0 kg ha-1, 20 kg ha-1, 50 kg ha-1, 100 kg ha-1). The screening of 100 kinds of medicinal herb, found that the inhibition of xanthine oxidase capacity of Origanum vulgare, Tetradenia riparia, Lavandula dentata, Tagetes lucida, Eucalyptus citriodora and Mentha x piperita are better than basil, which peppermint, sweet marigold, and oregano has been used in general diet. The results showed that the XO inhibition activities of peppermint, sweet marigold, oregano and sweet lavender was significantly correlated with normalized NDVI 705,750. The correlation between nitrogen fertilizer level and NDVI broad (660 ~ 705,750 ~ 800) was also significant. Therefore, when the production of medicinal herb can be quantified, the non-destructive plant index can be used to assist the management of nitrogen fertilizer and the ability of the raw material to inhibit xanthine oxidase can provide reference for good agricultural standard. In the future, can develop exquisite agricultural cultivation of natural health plants, to encourage cooperation in research and development with a unique product.

並列關鍵字

herb ； antioxidant activity ； XO inhibition activity ； leaf reflectance spectra

參考文獻

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