OPTIMIZATION OF BACTERIOCIN PRODUCTION BY LACTIC ACID BACTERIA FROM SPENT COFFEE GROUND HYDROLYSATE

Thangavel Ponrasu ； Chia-Wei Huang ； Yu-Shen Cheng

《Journal of Innovative Technology》 2卷1期 (2020/03) Pp. 1-8

https://doi.org/10.29424/JIT.202003_2(1).0001

摘要

The instant coffee industry produces a considerable amount of spent coffee ground (SCG) as a residue that could be potentially utilized as a renewable lignocellulosic biomass feedstock for the production of bacteriocin. Bacteriocins have attracted much attention from academia and industry because of their possible applications as non-toxic additives in food preservation and prevention of food spoilage from food-borne pathogenic bacteria. The objective of this study was to evaluate the possibility of producing bacteriocin by Lactobacillus plantarum BCRC 10069 and Lactobacillus paracasei LCW23 using SCG as feedstock. The bacteriocin produced by L. plantarum and L. paracasei showed significant inhibitory activity against Gram-positive (Bacillus cereus) and Gram-negative bacteria (Escherichia coli). Response Surface Methodology (RSM) was used to optimize the factors involving in bacteriocin production such as pH, glucose concentration, and cysteine concentration. The optimum conditions for bacteriocin production from L. plantarum were found to be 15 mg/mL of SCG concentration, 0.059% (w/v) of cysteine content, and pH 7.1; while the optimum conditions for L. paracasei were 19.1 mg/mL of SCG concentration, 0.062% (w/v) of cysteine content, and pH 7.08. Under the optimal culture conditions, the specific activities of produced bacteriocins could respectively reach 1163 AU/mg and 1920 AU/mg after 24 h of inoculation.

關鍵字

Bacteriocin ； Antimicrobial activity ； Lactobacillus plantarum ； Lactobacillus paracasei ； SCG

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