Universities in various places have started innovation and entrepreneurship education for college students, and trained applied talents with innovation and entrepreneurship ability. However, while the innovative and entrepreneurial ability of higher vocational college students has been greatly improved, there are still some difficulties such as insufficient entrepreneurial ability, low success rate and low employment quality. Studio mode is a relatively new way of college students' innovation and entrepreneurship, which can promote the process of college students' entrepreneurial practice. In view of the shortcomings of the innovation and entrepreneurship professional studio mode, this paper puts forward some countermeasures, such as the research on the construction of professional studio, the establishment of new school-enterprise cooperation mode such as enterprise studio, and the improvement of the evaluation system of innovation and entrepreneurship professional studio, with a view to gradually optimizing in theory and practice.