Profiling and determination of phenolic compounds in poly herbal formulations and their comparative evaluation

Sathis Kumar Dinakaran ； Shalaka Chelle ； Harani Avasarala

《Journal of Traditional and Complementary Medicine》 9卷4期 (2019/10) Pp. 319-327

https://doi.org/10.1016/j.jtcme.2017.12.001

摘要

Nowadays, plants have been considered as powerful agents for treatment of disorders regarding to their traditional use. Plants have a special role in the treatment of various diseases in Ayurveda (Indian Traditional Medicine). Diabetes with their devastating outcomes has been discussed in Ayurveda as well. In the present study, a marketed polyherbal products (DBC & DMV), retrieved from Ayurveda, was purchased from market and its pharmacognostic standardization were performed. Quality control test for the Ayurveda tablets were performed as per Indian Pharmacopoeia. In addition to the dissolution studies for the poly herbal Ayurveda marketed formulations were assessed based on the phenolic content. Fingerprinting of phytochemical constituents of DBC & DMV was performed using spectroscopical (like IR and UV) and chromatographic techniques like HPTLC and TLC. The results showed that DBC & DMV was successfully passed quality control tests. Moreover, DBC & DMV exhibited different pharmacognostic behavior of all herbs present in the product. In addition, TLC, IR and HPTLC fingerprinting of DBC & DMV demonstrated the presence of several phenolic constituents corresponding to the poly herbs. Regarding to the role of phenolic compounds in diabetic process, DBC & DMV could be an appropriate candidate for diabetic with respect to its traditional use in Ayurveda formulation. Moreover, HPTLC fingerprinting could be utilized as an applicable method for quality control of the prepared formulation.

關鍵字

HPTLC fingerprinting ； Ayurveda formulations ； Dissolution studies ； Standardisation ； Spectroscopical evaluation

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