Taking Ulva lactuca as the research object, the relative growth rate, photosynthetic pigment content, and chlorophyll fluorescence parameters of the photosynthetic organism with nitrogen enrichment were measured, to determine the function of nitrogen enrichment on the photosynthetic mechanism. The results showed that the fraction of OEC, ETo/RC, Fv/Fo, φPo, PIabs, φEo, ψo were significantly higher than those of control group (p<0.05). And Wk, Mo, DIo/RC and φDo were lower than those of control group (p<0.05), respectively. Nitrogen supplementation promoted the activities of donor side, recipient side and reaction center of Ulva lactuca on photosynthetic system II (PSII). In conclusion, nitrogen enrichment at 200μmol/L can effectively improve the growth rate and photosynthetic performance of Ulva lactuca, thus promoting its photochemical activity.