- 期刊
本土乳製品之優良乳酸菌篩選及其胞外多醣體產量比較
Isolation and culture of high exopolysaccharide producing lactic acid bacteria from native fermented milk
摘要
從台灣的乳製品中篩選得五株高產量胞外多醣（Exopolysaccharide，EPS）乳酸菌，分別鑑定為Lactobacillus delbrueckii subsp. bulgaricus、Leuconostoc spp.、Lb. rhamnosus、Lb. delb. lactis和Lb. paracasei。其中以Lb. delbrueckii subsp. bulgaricus EPS產量最高，而Lb. paracasei是首次由台灣乳製品中分離純化之高產量EPS乳酸菌，頗具研究及應用價值。5株菌在MEPS-2培養基中生長良好，生菌數及EPS產量較高。以試驗配方先以菌粉於37°C發酵24小時後，再加入篩選的乳酸菌移置於10°C培養48小時，所得EPS產量，較培養基同時放入篩選的菌株與菌粉於37°C培養24小時，再移置於10°C培養48小時者為佳。
並列摘要
Five species of high exopolysaccharide (EPS) producting lactic acid bacteria were isolated from local fermented milk and identified as Lactobacillus delbrueckii subsp. bulgaricus, Leuconostoc spp., L. rhamnosus, L. delb. lactis and L. paracasei. The L. delbrueckii subsp. bulgaricus had the highest EPS production assay among the five species bacteria. Above all, L. paracasei was the first one isolated from Taiwan milk products. The cultural studies showed that the better microbial counts and EPS products for five species of lactic acid bacteria were obtained from MEPS-2 medium at 15°C. Post-fermented milk culture medium at 37°C for 24 hours followed by one at 10°C for 48hours and single-strain starter culture had maximum EPS production.
延伸閱讀
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