台灣水牛在動情週期、懷孕期及產後45天期間之血清孕酮濃度變化

本試驗旨在探討台灣母水牛於動情週期、懷孕期及分娩後45天內之血清孕酮（progesterone,P_4）濃度變化，供為懷孕早期診斷及產後回復發情等之判定，並作為母水牛在繁殖配種及飼養管理等之參考。試驗使用不同年齡之母水牛16頭，分別於涼季（2月，n=10）及熱季（8月，n=6）連續測定2個動情週期中D0、D3、D7、D10、D14、D17及D20之血清P_4濃度（發情第一日為D0）。另外，涼季之母水牛於第三個動情週期以公牛進行自然配種，並每星期採血一次，以測定在懷孕期及分娩後45天內之血清P_4濃度。結果顯示，隨著動情週期之進行，血清P_4濃度亦隨之提高，並持續至D14達高峰，而於D17後P_4濃度即迅速下降，且在D7至D14間之血清P_4濃度以涼季者顯著較熱季者為高（P＜0.05）。配種懷孕之母水牛，其血清P_4濃度於D14後持續維持在動情週期之高峰濃度，在大多數母水牛懷孕早期（懷孕63日內）之血清P_4濃度均在1.5ng/mL以上，而未懷孕之母水牛D20的血清P_4濃度則下降至0.85ng/mL以下。初產母水牛在懷孕期之平均血清P_4濃度及高峰濃度顯著較經產母水牛為低（P＜0.05），但初產母水牛血清P_4濃度之上升速度較經產母水牛為快。母水牛在懷孕期前10個月之平均血清P_4濃度高於2.0ng/mL，而至分娩前5～10天之血清P_4濃度驟降至1ng/mL以下，而產後45天內之血清P_4濃度甚至低於0.25ng/mL以下。

關鍵字

台灣水牛；動情週期；季節；懷孕期；血清孕酮濃度

並列摘要

The purpose of this study was to investigate the variation of serum progesterone (P_4) concentration of the Taiwan female water buffaloes during estrous cycle, pregnancy and forty five days after parturition for early pregnancy diagnosis, post-parturition oestrus, and feeding and management reference. Sixteen female water buffaloes were randomly assigned to cool (February, n = 10) and hot (August, n = 6) season groups. The buffaloes were bled at D0 (the first day of estrus), D3, D7, D10, D14, D17 and D20 to measure serum P_4 concentration during two consecutive estrous cycles in hot and cool season. The buffalos in cool season group were mated by the bull at the third estrous cycle and were bled weekly to measure P_4 concentration during pregnancy and the first 45 days post-parturition. The results indicated that the serum P_4 concentration of buffalos increased with the advancement of estrous cycle. It reached peak at D14, and declined sharply at D17. However, the serum P_4 concentrations during D7 to D14 was significantly (P＜0.05) higher in cool season than those in hot season. The serum P_4 concentration of pregnant buffalos reached a plateau after D14 at the estrous cycle. The serum P_4 concentrations of most of the pregnant buffaloes were above 1.5 ng/ mL in the early stage of pregnancy (the first 63 days of pregnancy). The serum P_4 concentrations in non-pregnant buffaloes dropped to below 0.85 ng/mL at D20. Besides, the mean P_4 concentrations and the peak concentrations in primiparous cows were significantly（P ＜ 0.05）lower than those of the multiparous cows. But P_4 concentrations went up rapidly in the primiparous cows than the multiparous cows during pregnancy. During the first ten months of the pregnancy, the average concentrations of the serum P_4 was higher than 2.0 ng/mL and then plummeted to below 1 ng/mL five to ten days before parturition. At the first forty-five days postpartum, the average concentrations of serum P_4 was reduced to below 0.25 ng/mL.

並列關鍵字

Taiwan water buffaloes ； Estrous cycle ； Season ； Pregnancy ； Serum progesterone (P_4) concentration

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