台灣水鹿發情同期化及人工授精技術之建立

本研究旨在建立台灣水鹿之發情同期化與人工授精技術。試驗一係評估母台灣水鹿於CIDR發情同期化處理期間之血清中助孕酮（SP_4）濃度變化。結果顯示，母台灣水鹿於CIDR埋植之第0、8及12日，其SP_4濃度分別為1.97±0.05、8.27±4.47和6.15±1.66 ng/mL；而於相同時段之對照組母鹿（未CIDR埋植者），其SP_4濃度則分別為0.50±0.24、3.49±0.39和2.26±1.02 ng/mL。其中各分析時段之SP_4濃度均以CIDR埋植組母鹿明顯高於對照組母鹿（P＜0.05）。試驗二係評估經CIDR埋植搭配eCG發情同期化處理之母鹿發情率與自然配種懷孕率。結果顯示，母鹿經CIDR搭配eCG發情同期化處理後之發情率為85.7%（12/14），此12頭發情母鹿經公鹿配種後有10頭懷孕，且10頭懷孕母鹿均成功分娩仔鹿。試驗三的目的則在探討稀釋液種類對母台灣水鹿經冷藏精液人工授精後懷孕率之影響。結果顯示，母台灣水鹿經Tris-Yolk稀釋液稀釋後之精液人工授精後，其懷孕率（75.0%）與分娩率（75.0%）均與該母鹿經Citrate-Yolk稀釋液稀釋之精液人工授精者並無顯著差異（100.0%和100.0%）。綜合上述結果發現，利用CIDR搭配eCG發情同期化處理母台灣水鹿可得極高之發情率；發情母鹿經人工授精後之懷孕率及分娩率均高達88.9%。本試驗之結果，已成功建立台灣水鹿之發情同期化與人工授精技術。

關鍵字

台灣水鹿；血清助孕酮濃度；同期化發情；人工授精；精液稀釋液

並列摘要

The aims of this study were to establish the techniques of estrus synchronization and artificial insemination (AI) in Formosan Sambar. The purpose of experiment 1 was to evaluate the change of serum progesterone (P_4) concentration in the period of Formosan Sambar does estrus synchronized with an intravaginal controlled internal drug releasing (CIDR-type S). The P_4 concentrations of serum derived from CIDR treated does at day 0, day 8 and day 12 were 1.97 ± 0.05, 8.27 ± 4.47 and 6.15 ± 1.66 ng/mL, respectively, while the non CIDR-treated does (control group) were 0.50 ± 0.24, 3.49 ± 0.39 and 2.26 ± 1.02 ng/mL, respectively. The results indicated that the P4 concentrations of CIDR treated does were higher than those from the control does (P＜0.05) during the tested periods. The purposes of experiment 2 were to determine the estrus rate and pregnant rate of the Formosan Sambar does following estrus synchronized with CIDR plus eCG. The result showed that the estrus rate of estrus synchronized Formosan Sambar does was 85.7% (12/14). Moreover, ten of the twelve estrus does were pregnant after mating with Formosan Sambar stag, and gave birth to 10 kids. The purpose of experiment 3 was to investigate the effect of the semen extenders on the pregnant and parturient rate of estrus synchronized Formosan Sambar does after AI with cooled semen. The result showed that the pregnant rate (75.0 vs. 100.0%) and parturient rate (75.0 vs. 100.0%) of estrus synchronized Formosan Sambar does after AI with cooled semen diluted with Tris-Yolk extender were not significantly different from those does after AI with cooled semen diluted with Citrate-Yolk extender. In conclusion, the estrus rate was 85.7% in Formosan Sambar does estrus synchronized with CIDR plus eCG, and the pregnant rate and parturient rate of estrus synchronized Formosan Sambar does after AI with cooled semen were 88.9%, The respective results of this study showed that the techniques of estrus synchronization and artificial insemination in Formosan Samba were well established.

並列關鍵字

Formosan Sambar deer ； Serum progesterone concentration ； Estrus synchronization ； Artificial insemination ； Semen extender

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