液相層析串聯式質譜儀檢測牛乳中頭孢菌素

Determination of cepharosporins in milk by liquid chromatography tandem mass spectrometry

鄧名志(Ming-Chih Teng) ； 李免蓮(Mian-Lian Lee)

《畜產研究》 43卷2期 (2010/06) Pp. 143-151

摘要

本研究係以具選擇性及靈敏度之高效率液相層析串聯式質譜儀（Liquid chromatography/tandem mass spectrometry, LC/MS/MS）搭配固相萃取（Solid phase extraction, SPE），建立牛乳中頭孢菌素殘留量之分析方法。使用三段式四極棒質譜儀搭配渦輪噴灑離子源在陽離子模式下偵測頭孢菌素。牛乳中頭孢菌素分析之方法偵測極限（Method detection limit, MDL）為0.74～2.44 ng/mL（ppb），於牛乳中添加10 ng/mL頭孢菌素，回收率69.84%～99.43%，變異係數（CV）值2.90%～8.52%。本方法可應用於牛乳中頭孢菌素殘留量分析。

關鍵字

牛乳 ； 頭孢菌素 ； 高效率液相層析串聯式質譜儀

並列摘要

The purpose of this study was to develop a specific and sensitive high performance liquid chromatography tandem mass spectrometry (LC/MS/MS) method with solid-phase extraction (SPE) for determination of cepharosporins residues in milk. A triple-quadruple mass spectrometry using a TurboIon Spray source operating in the positive ion mode was used to detect cepharosporins. The method detection limit (MDL) for cepharosporins were 0.74 ~ 2.44 ng/mL. At 10 ppb level in milk, recoveries of cepharosporins were between 69.84 and 99.43%, with coefficient of variation (CV) ranging between 2.90 and 8.52 %. The method can be applied to the analysis of cepharosporins residues in milk.

並列關鍵字

Milk ； Cepharosporins ； High performance liquid chromatography tandem mass spectrometry