雞始基生殖細胞移植後之胚胎存活率與其性腺遷徙之探討

始基生殖細胞（primordial germ cell, PGC）為精子與卵子的前驅細胞，是目前家禽基因轉殖研究甚具潛力之轉殖途徑之一。本研究之目的是探討家禽PGC進行活體移植後之胚胎存活率及性腺遷徙效率。PGC的收集是自孵化5.5天的來亨雞早期胚胎之性腺進行分離而得；在評估移植後之胚胎存活率與性腺遷徙效率，是先將PGC以電穿孔方式轉染綠色螢光蛋白質（green fluorescent protein, GFP）報導基因後，注入2μL（150 － 500 cells ／ μL）的PGC到孵化3.5天（stage 12 － 15）胚胎的背大動脈之方式進行活體移植測試。結果顯示，胚胎進行PGC移植後，在孵化到第10天和19天的存活率分別為68.7%（90／131）和60.0%（39／65）；在移植後孵化到第10天和19天胚胎性腺GFP報導基因的檢測呈現陽性反應分別為20.7%（38／183）和11.4%（7／61）。

關鍵字

始基生殖細胞；雞；活體移植；胚胎存率；性腺遷徙率

並列摘要

Primordial germ cells (PGCs) are the precursors of the germ cell lineage and are restricted to the formation of sperm and eggs in the adult organism. PGCs are recognized as the most potential pathway for the production of transgenic avian. The objective of this study is to evaluate the embryo survival rate and gonadal migration efficiency of PGCs after transplantation. Chicken PGCs collected from the primitive gonads of White Leghorn chicken embryos (5.5-day-old) were used as donor cells for transplanting into the recipient chicken embryos. Before transplantation, an eGFP reporter gene was transferred into the PGCs. Approximately 2μL of electroporated PGCs suspension containing 150-500 cells μL were injected into the dorsal aorta of the recipient chicken embryos (3.5-day-old). The results showed that the survival rate of the treated embryos at day 10 and 19 after incubation was 68.7% (90/131) and 60.0% (39/65), respectively. The expression of eGFP gene was detected in the gonads of 20.7% (38/183) and 11.4% (7/61) embryos examined at days 10 and 19 of incubation, respectively.

並列關鍵字

Primordial germ cell ； Chicken ； In vivo transplantation ； Embryos survival rate ； Gonadal migration efficiency

國際替代計量

雞始基生殖細胞移植後之胚胎存活率與其性腺遷徙之探討

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