- 學位論文
活體觀察性腺組織與細胞的生殖保存與移植-基因轉殖鼠模式
Evaluation of gonadal tissue and cells in vitro and transplantation in vivo for fertility preservation -a transgenic mouse model
摘要
癌症治療的進步改善了年輕癌症患者的長期生存。生育能力保存於 生殖功能受到威脅的情況下已成為下一個新興的需要和關注的問 題。放療和化療對性腺功能的影響與治療方法引起的發病率有關。 目前在臨床上常見使用的烷化劑是最可能引起性腺毒性的化療藥 物。持續努力和關注年輕婦女癌症病患，保留生育功能對男女雙方 都非常重要的。隨著90 年代以來，卵巢組織冷凍保存在癌症治療完 成後的後續移植進行的研究進展顯著增加已建議作為替代，以恢復 在化療或放療後卵巢衰竭的年輕婦女的生育。卵巢組織在同系或同 種是可能的，在同系或同種排斥的問題並不清楚。 睪丸精原細胞含具有造精能力的幹細胞，青春期前癌童接受放療和 化療的應用前景為保存及移植睪丸精原幹細胞以恢復生育能力。 生物冷光活體(In vivo bioluminescence imaging)研究系統對於組織或 器官的移植、存活及排斥等非侵入式的觀察具有諸多優點，包含減 少無法在體外模擬的環境變因以及在活體內進行即時而長期的動態 追蹤等。本研究利用基因轉殖冷光小鼠生物影像技術以非侵入的方 式研究，不會影響到細胞或是任何組織器官就能取得體內更令人信 服的定性與定量資料，非一般體外及傳統體內研究方式所能模擬。
並列摘要
Advances in cancer treatment may improve long-term survival of young cancer patients. The preservation of fertility has become an emerging needs and issues of concern threatening situations anywhere in the reproductive function. The impact of radiotherapy and chemotherapy on gonadal function, morbidity is caused to lost fertility function. Currently, in common clinical use of alkylating agents is the most likely cause gonadal toxicity of chemotherapy drugs. Maintain the effort and attention in the fertility of young female cancer patients. To preserve fertility in both men and women are very important. With Since the 1990s, the ovarian tissue frozen to save the progress of research in the follow-up after transplantation in cancer treatment is completed to a significant increase has been proposed as an alternative to restore the fertility of ovarian failure after chemotherapy or radiotherapy, high-risk girls and women. Ovarian tissue transplantation in the isografts and allografts is a possible application in human but the rejection and survival remains unclear. Testes spermatogonial cells containing stem cells of spermatogenesis, prepubertal cancer children to accept the application prospects of for preservation and transplantation of testes spermatogonial stem cells to restore fertility after radiotherapy and chemotherapy. Bioluminescence living (In vivo bioluminescence imaging) research system for tissue or cell transplantation, survival and rejection and other non-invasive observation has multi-advantages, contains reducing unable to in vitro simulation of the environment variable due as well as in vivo into the conduct real-time while long-term dynamic tracking. In this study, using transgenic mice carrying transgene as a donor evaluate bio-imaging technology in small animal model to a non-invasive manner, will not affect the cells or tissues. Compel qualitative and quantitative data, non-general in vitro and traditional in vivo studies way that can be simulated.
參考文獻
延伸閱讀
- 劉振發、許義明、劉曉龍、郭曉芸、蕭振文、戴謙、陳立人（2015）。雞始基生殖細胞移植後之胚胎存活率與其性腺遷徙之探討。畜產研究，48(1)，77-85。https://www.airitilibrary.com/Article/Detail?DocID=P20200930001-201503-202010230015-202010230015-77-85
- 陳冠燁（2008）。無患子體胚誘導之研究〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2008.03254
- Wang, C. J., & Howng, S. L. (1996). 細胞培養中腦垂促乳素腺瘤構造功能間之相關性及早期再發之研究. The Kaohsiung Journal of Medical Sciences, 12(7), 394-399. https://doi.org/10.6452/KJMS.199607.0394
- Anguiano, V. B., Suárez, S. O., Vences, E. G., Chacón, S. C., Sahagún, G. G., Sanchez, L. C., & Grijalva, I. (2015). The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection. Neural Plasticity, 2015(), 558-569-047. https://doi.org/10.1155/2015/389520
- Lee, J. L. (2020). Time-course Study of in vivo and in vitro Nanoparticle Translocation in Mice [master's thesis, Chung Yuan Christian University]. Airiti Library. https://doi.org/10.6840/cycu202000644