To assess the reliability behavior of a typical TSV structure, this study performed reliability tests on self-design samples with three types of the TSV test-keys, which includes the Kelvin structure, the via-chain structure, and the meander metal lines. With enough number of the samples for statistic analyses, resistance of the test samples were first found increased after the preconditioning process. The reliability tests were next carried out according to the JEDEC standards, and the Weibull parameters for the testing samples were then extracted from the testing data to predict the lifetime performance on the samples. Finally, OM and SEM observations on the test samples are given for failure analyses.