發酵溫度影響Bacillus subtilis BCRC 14715發酵黑豆中機能性成分之改變

Fermentation Temperature Affects the Alteration of Functional Constituents in Bacillus subtilis BCRC 14715-fermented Black Soybean

吳佳璇(Chia-Hsuan Wu)

指導教授 ： 周正俊

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2008年)

https://doi.org/10.6342/NTU.2008.00366

摘要

本研究以黑豆為原料，接種Bacillus subtilis BCRC 14715進行固態發酵，探討不同發酵溫度 (35, 40, 45, 50°C) 於培養期間 (0, 6, 12, 18小時) 對黑豆中一些活性成分，包括異黃酮素、納豆激酶 (nattokinase) 、維生素K2、血管收縮素轉化酶抑制劑 (angiotensin- converting enzyme inhibitor, ACE inhibitor) 、超氧歧化酶 (superoxide dismutase, SOD) 及吡啶二羧酸 (dipicolinic acid, DPA) 變化之影響。
結果顯示，40及45°C為B. subtilis BCRC 14715在黑豆基質中之最適生長溫度。無論發酵溫度為何，黑豆之總異黃酮含量在發酵過程中皆逐漸降低，其中以在35℃下發酵者下降之趨勢最為顯著。經發酵後之黑豆，其醣苷配基 (aglycone) 形式之異黃酮素含量，隨發酵時間增長而有明顯上升之趨勢 (p＜0.05)，其中以在50°C下發酵之黑豆可獲得最高aglycone形式之異黃酮素。在各發酵溫度下，黑豆中β-葡萄醣苷酶 (β-glucosidase) 活性皆於發酵6小時達最大值，其後即呈下降之趨勢。此外，隨發酵時間延長，黑豆中納豆激酶活性、維生素K2含量、ACE inhibitor活性、SOD活性及DPA含量均逐漸提升。黑豆經發酵18小時後，以在35°C下發酵者可得最高之納豆激酶活性，維生素K2則是在45°C下發酵可獲得最大產量；黑豆於40及45°C下發酵可呈現較佳之ACE抑制活性及SOD活性；在50°C下發酵者，則可獲得最大之DPA產量。

關鍵字

Bacillus subtilis ； 異黃酮 ； 納豆激酶維生素K2 ； 血管收縮素轉化酶抑制劑 ； 超氧歧化酶吡啶二羧酸

並列摘要

In the present study, fermented black soybean was prepared with Bacillus subtilis
BCRC 14715. The changes of some functional constituents of fermented black soybean, including isoflavone, nattokinase, vitamin K2, angiotensin- converting enzyme inhibitor (ACE), superoxide dismutase (SOD), and dipicolinic acid (DPA), during the fermentation (0, 6, 12, 18 h) with B. subtilis BCRC 14715 at various temperatures (35, 40, 45, 50°C) were investigated.
The results revealed that the optimum growth temperatures of B. subtilis BCRC 14715 are 40－45°C in black soybean. The total content of isoflavone in black soybean decreased during fermentation, regardless of fermentation temperatures. The most marked change was observed at 35°C. Fermentation caused a significant increase (p＜0.05) in the content of aglycone, which enhanced with fermentation time. The highest level of aglycone was obtained in black soybean fermented at 50°C. Regardless of fermentation temperatures, the activity of β-glucosidase noted in black soybean reached the highest after 6 h of fermentation and then decreased progressively. In addition, nattokinase activity, vitamin K2 content, ACE inhibitor activity, SOD activity and DPA content of black soybean increased as the fermentation time was extended. Black soybean fermented at 35°C for 18 h exhibited the highest level of nattokinase activity, while the highest content of vitamin K2 and DPA was found in black soybean fermented at 45 and 50°C, respectively. On the other hand, a higher activity of ACE inhibitor and SOD was observed in black soybean fermented at 40 and 45°C.

並列關鍵字

Bacillus subtilis ； isoflavone ； nattokinase ； vitamin K2 ； angiotensin- converting enzyme inhibitor ； superoxide dismutase ； dipicolinic acid

參考文獻

林文彬。2005。納豆製造及養身應用。傳統醫學雜誌。16: 8-23。

施明智、楊開聰、郭淑姿。2006。黑豆納豆發酵過程理化性質之變化。台灣農業化學與食品科學。44: 382-389。

莊荃與、林頎生、謝寶全。2006。Bacillus subtilis B-12 深層發酵生產超氧歧化酶最適化之探討。台灣農業化學與食品科學。44: 345-350.

楊棋明、楊智旭、趙碧玉。1999。市售黑豆與黃豆抗氧化力之灰預測比較。中華民國營養學會雜誌 24: 201-214。

廖文進、吳錦楨、何善台、王志中。2008。自由基與敗血症：超氧歧化酶的角色。重症醫學雜誌。9: 10-19。