利用全域式光學同調斷層掃描術於角膜神經在疾病模式下的量化分析

Quantification of Corneal Nerve’s Disease Model Images Using Full-Field Optical Coherence Tomography

廖家沛(Chia-Pei Liao)

指導教授 ： 黃升龍

共同指導教授 ： 陳偉勵(Wei-Li Chen)

國立臺灣大學/電機資訊學院/光電工程學研究所/碩士(2022年)

https://doi.org/10.6342/NTU202203268

摘要

在角膜的研究與檢測中，建構高解析度的三維角膜影像有助於提供臨床醫生得到更多角膜診斷資訊，如在縱切面上得到各層厚度、在橫切面得到各層結構內部形貌以及角膜亞基底神經叢形貌變化。角膜是具有大量感覺神經的組織，目前許多文獻顯示許多的眼表面及系統性疾病皆與角膜亞基底神經叢形貌變化有關，例如：糖尿病Ⅰ、Ⅱ型與帕金森氏症。因此精確定量角膜亞基底神經形貌能為早期診斷提供幫助。
在本研究中，使用等向性之微米級空間解析度的可見光Ce3+:YAG自發輻射與近紅外光Ti:sapphire自發輻射兩套光源之全域式光學同調斷層掃描術分別對檢體與活體來展示角膜各層結構之橫平面與縱平面以及三維立體影像。
藉由健康與受傷角膜模式之檢體小鼠實驗，證實本系統具有細胞等級的成像能力，並且與自行撰寫之曲面擬合與投影程式及前人的TCCMetrics結合能夠得到更完整的角膜亞基底神經影像並做精確的定量分析，各個健康與損傷角膜神經參數的差異以及復原趨勢大致符合生理學。具體而言，健康與受傷角膜模式之檢體小鼠結果對比為：健康的主幹神經密度為136.93 ± 19.18 / 〖mm〗^2，損傷模式角膜之主幹神經密度在復原時間第52天為大約70 / 〖mm〗^2，是健康的神經的50%左右；健康的亞基底神經密度為61.46 ± 1.78 mm / 〖mm〗^2，損傷模式角膜為大約15 mm / 〖mm〗^2，是健康神經的24%左右；健康分枝神經密度為 731.00 ± 186.19 / 〖mm〗^2，損傷模式角膜為大約40 / 〖mm〗^2，不到健康神經的10%；分枝神經連接點密度為 608.01 ± 116.99 / 〖mm〗^2，損傷模式角膜為大約40 / 〖mm〗^2，不到健康神經的10%；健康平均分枝點為 4.57 ± 1.41，損傷模式角膜為大約0.5，大約為健康神經的10%；分枝主幹比為 5.53 ± 2.04，損傷模式角膜為大約0.5，大約為健康神經的10%。

關鍵字

全域式光學同調斷層掃描 ； 角膜 ； 角膜亞基底神經 ； 神經形貌量化

並列摘要

In corneal research and examination, high-resolution three-dimensional corneal images can help clinicians to obtain more corneal diagnostic information, such as the thickness and the internal morphology of each layer and the corneal sub-basal nerve. The cornea is a densely innervated tissue with sensory nerve fibers, and many literatures have shown that both ocular surface and systemic diseases are associated with corneal nerves, such as diabetes and Parkinson's disease. Therefore, accurate quantification of corneal nerve can provide help for early disease diagnosis.
Here we develop Ce3+:YAG and Ti:sapphire light source full-field optical coherence tomography (FF-OCT) systems with isotropic micron spatial resolution to get the en-face, cross sectional views and three-dimensional images from ex vivo and in vivo samples.
We validated the imaging ability with healthy and injured models of ex vivo mice, and get the accurate quantitative analysis from more complete corneal sub-basal nerve images using curve plane tracing and projection code and TCCMetrics. The comparison between quantitative results of ex vivo healthy and injured models mice are as follows: NFD, healthy: 136.93 ± 19.18 / 〖"mm" 〗^"2" , injured: 70 / 〖mm〗^2; NFL, healthy: 61.46 ± 1.78 mm/〖"mm" 〗^"2" , injured: 15 mm / 〖mm〗^2; BND, healthy: 731.00 ± 186.19 / 〖"mm" 〗^"2" , injured: 40 / 〖mm〗^2; BNCD, healthy: 608.01 ± 116.99 mm/〖"mm" 〗^"2" , injured: 40 / 〖mm〗^2; BNCM, 4.57 ± 1.41, injured: 0.5; BMR, healthy: 5.53 ± 2.04, injured: 0.5; NFW, TC, K line, numbers of short nerve fiber, parallelism and standard deviation of single-image parallelism have no significant difference.

並列關鍵字

Full-field optical coherent tomography ； Cornea ； Corneal sub-basal nerve ； Quantitative of corneal sub-basal nerve

參考文獻

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