以固相微萃取技術發展空氣中戊二醛之被動式採樣

Passive Air Sampling for Glutaraldehyde by Solid Phase Microextraction

雷蕙禎(Huei - Jen Lei)

指導教授 ： 林嘉明

國立臺灣大學/公共衛生學院/環境衛生研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.00579

摘要

本研究應用五氟芐羥胺〔O-(2,3,4,5,6-pentafluorobenzyl)- hydroxylamine hydrochloricde (PFBHA)〕裹附於polydimethylsiloxane/ divinylbenzene (PDMS/DVB)纖維上，然後再與戊二醛反應生成戊二醛-PFBHA oxime，希望結合固相微萃取技術發展空氣中戊二醛之被動式採樣，並且探討固相微萃取 (Solid Phase MicroextractionֽSPME) 之被動式採樣的表現。
透過實驗室架設的標準氣體產生系統，控制戊二醛濃度、溫度及相對溼度，並且配合SPME之裝置，調整不同的擴散徑長，形成被動式採樣設備，設定不同的採樣時間以進行氣態戊二醛的採樣。待樣本收集完畢後，直接插入配有電子捕獲偵檢器之氣相層析儀注射口中，進行樣本的脫附與分析。
結果顯示，PDMS/DVB纖維在三種擴散徑長下其吸附量對戊二醛濃度與時間的乘積有良好的線性相關 ( r > 0.90；p < 0.05 )；溫度(15℃、25℃、30 ℃)及溼度(0%、50%、80%)並不影響採樣表現。影響採樣率的最重要變項為擴散徑長，影響程度為88.36%。三種擴散徑長下所得到的採樣率分別為3mm: 0.0137 ±0.0009 mL/min、10mm: 0.0062±0.00144 mL/min以及 15mm: 0.0036 ±0.0005 mL/min。

關鍵字

戊二醛 ； 被動式採樣 ； 固相微萃取 ； 五氟芐羥胺〔O-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine hydrochloricde (PFBHA)〕 ； polydimethylsiloxane/ divinylbenzene (PDMS/DVB)

並列摘要

This study evaluated the sampling performance of the SPME device as a passive sampler. O-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine hydrochloricde, PFBHA was loaded onto the polydimethylsiloxane/ divinylbenzene (PDMS/DVB) fiber for the determination of gultar- aldehyde with on-fiber derivatization.
A laboratory standard gas generation system was built to regulate the concentration of glutaraldehyde, temperature and humidity in the exposure chamber. SPME assemblies were set at different length of diffusive paths to collect airborne glutaraldehyde for different time periods. After sampling, the sampler was inserted into the injection port of gas chromatography with a electron capture detector for thermal desorption and sample analysis.
The results show that the amount of glutaraldehyde collected on PDMS/DVB fiber is highly correlated with the product of concentration of glutaraldehyde and sampling time (r > 0.90 ; p < 0.05) while temperature (15℃,25℃,30 ℃) and relative humidity (0%,50%,80%) did not show any significant effect on the SPME passive sampling. The one predominant factor to influence the sampling rate is the length of diffusive path (88.36%). And when the diffusive paths were 3 mm, 10 mm and 15 mm, the sampling rates were 0.0137 ± 0.0009 mL/min, 0.0062± 0.00144 mL/min and 0.0036 ± 0.0005 mL/min, respectively.

並列關鍵字

glutaraldehyde ； SPME ； PFBHA ； PDMS/DVB

參考文獻

37.黃敬德，謝有容，固相微萃取技術之原理及其應用，化學，1998，56(4): p. 311-318.

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