摻偽蜂蜜理學辦別指標之建立

The Establishment of Physicochemical Index for Honey Adulteration

蔡心慈(Shin-Tzu Tsai)

指導教授 ： 謝博全

國立臺灣大學/生物資源暨農學院/生物產業機電工程學研究所/碩士(2018年)

https://doi.org/10.6342/NTU201803162

摘要

蜂蜜摻偽是食品摻假常見的議題，然而目前國際公認且通用的檢測蜂蜜摻偽技術—「穩定碳同位素值比分析」，需依賴昂貴的質譜儀，且檢測程序繁瑣及耗時。因此，本研究旨在分析辨別蜂蜜摻偽之理學指標，以建立輔助官方檢測的方法，使官方檢測程序更加便捷。本研究共收集26件調和蜜、48件純龍眼蜜以及23件純荔枝蜜，透過物理化學性質分析以及次要化學物質定量，獲得糖度、pH值、導電度、顏色強度（A450-A720）與濁度（A720）、脯胺酸含量及葡萄糖酸含量等七個數據，並將數據作為檢測蜂蜜摻假的指標，進行統計分析。實驗結果顯示，糖度對蜂蜜摻偽無區別性。顏色強度及pH值等作為檢測蜂蜜摻假的指標不適用。以濁度、導電度、脯胺酸含量以及葡萄糖酸含量等皆可作為檢測蜂蜜摻假的指標。透過本研究結果得知，可藉由蜂蜜樣品濁度小於0.0585者；或是導電度小於170.3 µS/cm者；或是脯胺酸含量小於180 ppm者；或是葡萄糖酸含量小於93.12 nmol者判定為調和蜜，並將篩選出的蜂蜜樣品以官方檢測法進一步分析，達到成本降低、省時便捷之目標。

關鍵字

蜂蜜摻偽 ； 理學指標 ； 物化特性 ； 次要化學物質 ； 便捷

並列摘要

Honey adulteration is a topic issue in the field of food authenticity. “Stable Carbon isotope ratio analysis” is the official method which used for detection of honey adulteration. However, this method is expensive, time-consuming and requires considerable analytical skills. Therefore, this study aims to analyze the physicochemical indexes for honey adulteration and aid the official method. The study collected 26 adulterated honeys, 48 pure Longan honeys and 23 pure Litchi honeys. In this study, the experiments were divided into two parts to acquire indexes for honey adulteration, which were (i) the analysis of physicochemical properties of honey and (ii) the quantitative analysis of minor chemical components of honey. The indexes included sugar content, pH value, electrical conductivity, color intensity (A450-A720), turbidity (A720), proline content and gluconate content. And the results showed that sugar content is indistinguishable. In addition, color intensity and pH value are not suitable for detecting honey adulteration. Turbidity, electrical conductivity, proline content and gluconate content can be used as indexes for honey adulteration. According to the results of this study, there are a few ways to determine the adulterated honeys which the turbidity of the honey sample is less than 0.0585; or the electrical conductivity is less than 170.3 μS/cm; or the content of proline is less than 180 ppm; or the content of gluconic acid is less than 93.12 nmol. And then, the honey samples are further analyzed by the official method to achieve the cheaper, more convenient and fast way for honey adulteration.

並列關鍵字

Honey adulteration ； Physicochemical index ； Physicochemical properties ； Minor chemical components ； Convenient and fast

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