蜂王漿與蜂子之血管緊縮素轉化酶抑制胜肽的研究

Study of the angiotensin I converting enzyme inhibitory peptides from royal jelly and bee larva

陳惠媛(Huei-Yuan Chen)

指導教授 ： 鄭光成

共同指導教授 ： 劉嚞睿(Je-Ruei Liu)

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2015年)

https://doi.org/10.6342/NTU.2015.01593

摘要

本研究目的欲以蜂王漿及蜂子之水溶性蛋白，以人體腸胃道酵素（胃蛋白酶、胰蛋白酶）進行水解，探討水溶性蛋白及其水解物對血管緊縮素轉化酶之抑制活性。分析不同水解時間下的水解程度及血管緊縮素轉化酶抑制活性。結果顯示，蜂王漿蛋白使用胃蛋白酶水解36小時後，水解度為60.63%，其血管緊縮素轉化酶抑制率為84.88%；而胰蛋白酶水解36小時後水解度為89.66%，其血管緊縮素轉化酶抑制率為94.02%。蜂子蛋白組別，使用胃蛋白酶水解36小時後，水解度為50.88%，其血管緊縮素轉化酶抑制率為95.12%；而胰蛋白酶水解36小時後水解度為50.75%，其血管緊縮素轉化酶抑制率為97.35%。再取各組水解36小時之水解液經不同分子量之濾膜區分（MWCO 5,000及 2,000），其中以蜂子蛋白經胰蛋白酶水解36小時的組別有最佳的抑制活性，分子量在> 5,000、2,000~5,000及2,000以下之血管緊縮素轉化酶抑制能力（IC50）分別為1.53、1.08及0.67 mg/mL。可以看出隨著分子量越小，有更好的抑制活性。再以帶電荷性質的差異，通過弱陰離子交換管柱（DEAE column）加以區分，由兩種樣品所得到抑制活性（IC50）最佳的兩個劃分（蜂王漿：0.32 mg/mL； 蜂子：0.17 mg/mL）再經高效能液相層析儀進一步純化。綜合以上結果可知，蜂王漿與蜂子蛋白在經過一系列水解、純化的步驟，可製備具有降血壓活性之短鏈胜肽，具有開發成健康食品之潛力。

關鍵字

蜂王漿 ； 蜂子 ； 高血壓 ； 血管緊縮素轉化酶抑制胜肽

並列摘要

In this research, water soluble protein of RJ and bee larva will be extracted and then hydrolyzed in vitro by gastrointestinal enzymes, pepsin and trypsin. After enzymatic treatment for 36 h, the angiotensin converting enzyme (ACE) inhibitory activities of the protein hydrolysates were evaluated. The results revealed that: in RJ group, that the degree of hydrolysis (DH) of the hydrolysate by pepsin for 36 h was 60.63% and the ACE inhibitory activity was 84.88%. While the DH of the hydrolysate by trypsin for 36 hours was 89.66 % and the ACE inhibitory activity was 94.02%. As for the bee larva group, the DH of the hydrolysate by pepsin for 36 h was 50.88% and the ACE inhibitory activity was 95.12%. While the DH of the hydrolysate by trypsin for 36 hours was 50.75% and the ACE inhibitory activity was 97.35%. Furthermore, the bioactive peptides in RJ and bee larva protein hydrolysate were purification by microfiltration (MWCO 5,000 and 2,000) and anion ion-exchange column (DEAE column). Two active fractions (IC50 value are 0.32 and 0.17 mg/mL) derived from royal jelly and bee larva which hydrolyzed by trypsin were purification and desalted by high performance liquid chromatography. These results indicated that, after hydrolyzed with protease, royal jelly and bee larva both have potential to develop antihypertensive functional foods.

並列關鍵字

royal jelly ； bee larva ； hypertension ； ACE-inhibitory peptides

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