蜂王幼蟲及其酵素水解產物之抗氧化特性與血管收縮素轉化酶抑制活性研究

Antioxidant Properties and Angiotensin I converting Enzyme Inhibitory Activity of Queen Bee Larva Protein Hydrolyates

鄭金源(Chin-Yuan Cheng)

指導教授 ： 彭及忠

國立虎尾科技大學/電機資訊學院/光電與材料科技研究所/碩士(2013年)

摘要

蜂王幼蟲又稱為蜂王胎，是為蜂農製備蜂王漿時的副產品，幼蟲在生長過程中完全食用營養豐厚的蜂王漿，蟲體成份與蜂王漿成份相近；除蜂王漿的成份外，尚有多種生物酵素及昆蟲的生長發育激素等營養物。隨著保健養生的意識抬頭，具有改善身體機能的各種保健食品日漸地受到民眾的重視，以期藉由保健食品來達到預防疾病之發生。蜂王胎的營養素含量多元且豐富，有利於用來進行保健食品的開發與研究。因此本實驗利用蜂王幼蟲作為研究對象，探討蜂王幼蟲經酵素水解後之產物的抗氧化特性與血管收縮素轉換酶抑制活性分析。本研究利用四種不同的水解酵素(trypsin、papain、bromelain、chitosanase)對蜂王幼蟲凍乾粉末進行酵素水解反應，並分別針對經四種酵素水解後所獲得的產物樣品進行研究與分析。實驗結果顯示蜂王幼蟲經酵素水解後的產物具有良好的抗氧化活性與血管收縮素轉換酶抑制活性。

關鍵字

蜂王幼蟲 ； 蜂王胎 ； 抗氧化 ； ACE

並列摘要

Queen bee larva, a by-product during the royal jelly production was used as a material in this study. First, the queen bee larva was lyophilized and then enzymatic hydrolysis by four enzymes (papain, trypsin, bromelain and chitosanase). We had evaluated the antioxidatives properties (DPPH radical scavenging activity, hydroxyl radical activity, scavenging activity of superoxide anion radicals, inhibition of linoleic acid peroxidation and reducing power) and angiotensin I converting enzyme inhibitory activity of enzymatic hydrolysates. The yield of these hydrolysates was very high, about 27-32% on a raw weight basis. The results demonstrate that enzymatic hydrolysates of queen bee larva protein possess high antioxidant activity and contain bioactivity angiotensin I-converting enzyme inhibitory activity. The enzymatic hydrolysates of queen bee larva could be a medicinal food in the human body.

並列關鍵字

queen bee larva ； antioxidant ； angiotensin I-converting enzyme ； hydrolysates

參考文獻

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[25] Nagai T., Inoue R., Suzuki N., Nagashima T. (2006) Antioxidant properties of enzymatic hydrolysates from royal jelly. Journal of medicinal food, 9: 363-367

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