利用電控微流體平台合成且圖案化異質且跨尺度之微組件

Heterogeneous and Cross-scale Microcomponent Synthesis and Patterning on an Electro-microfluidic Device

陳奕伶(Yi-Ling Chen)

指導教授 ： 范士岡

國立臺灣大學/工學院/機械工程學研究所/碩士(2016年)

https://doi.org/10.6342/NTU201603471

摘要

本研究主要利用電控微流體 (Electro-microfluidic) 操控技術建造、排列水膠微組件，並利用可自定義圖形之 UV 光源將水膠圖案化。實驗中以介電濕潤 (Electrowetting on dielectric，EWOD) 現象為移動水膠液滴之方法，藉由設計之電極圖案，操控含有不同螢光粒子的水膠液滴，並將之排列為不同大小以及不同圖案的水膠微結構。生物組織之技術已發展的相當成熟，藉由建造微組件並將之排列成三維結構也有許多合成方式，例如：微囊胞形成法、光刻法、微塑形法以及微流道方法等，但各種方法都有其限制。實驗中主要使用聚乙二醇二丙烯酸酯(Poly(ethylene glycol) diacrylate，PEGDA) 之水膠材料，將 PEGDA 與螢光粒子混合成水膠溶液 (100% PEGDA 與 0.5% 光起始劑) 後，在高度 100 μm 的電控微流體平台中，以 20-80 Vpp，1 kHz 的交流電訊號操控水膠溶液。利用介電濕潤現象形成、分離及移動液滴。將液滴移動至特定位置後，以可圖案化之 UV光將水膠溶液定義成特定圖形。透過操控含有兩種不同螢光粒子的水膠溶液，我們已在電控微流體平台上成功合成出 1 × 3、2 × 2、2 × 3 及 3 × 3 之水膠微組件陣列，也成功的建構出十字形狀、螺旋形狀以及六角形的異質結構，並於電控微流體平台上操控固態之水膠微組件。未來可將生物材料加入水膠結構當中，應用於組織工程。

關鍵字

異質結構 ； 水膠 ； 介電濕潤 ； 電控微流體平台

並列摘要

We synthesized and assembled the hydrogel microcomponents on an electro-microfluidic device with patterned UV illumination. Electrowetting on dielectric (EWOD) was adopted to manipulate the hydrogel prepolymer droplets with fluorescent particles on the designed electrodes and to assemble the crosslinked hydrogel microcomponents into a programmalbe microstructure. We used Poly(ethylene glycol) diacrylate (PEGDA) with photoinitiator (concentration 0.5 (w/v)%) as the hydrogel material, and mixed it with fluorescent particles as the manipulating prepolymer solution. On applying 20-80 Vpp and 1 kHz AC signals, we transferred, merged and split the hydrogel solution droplets between the plates (gap height 40 μm) on the electro-microfluidic device with EWOD. Hydrogel microcomponents were prepared through (1) transferring and assembly of multiple droplets on a specific location and (2) crosslinking with patterned illumination. By assembly the crosslinked hydrogel microcomponents containing varied and reorganized fluorescent particles, we successfully constructed heterogeneous hydrogel structures with 1 × 3, 2 × 2, 2 × 3 and 3 × 3 microcomponents and cross-, spiral- and hexagonal- shaped heterogeneous structure. To construct heterogeneous hydrogel structure, the cross-scale patterned hydrogel microcomponents were manipulated in another hydrogel material, and cured in different pattern. In the future, we will replace the material with cell or biomaterial to apply on tissue engineering and biomedics.

並列關鍵字

heterogeneous hydrogel ； electrowetting ； electro-microfluidic device

參考文獻

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